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EMAGE:4130

Mbnl3 muscleblind-like 3 (Drosophila) ( MGI:2444912)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:4130
Figure 2A. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S1567-133X(03)00064-4] Gene Expr Patterns 3: 459-62, Kanadia RN; Urbinati CR; Crusselle VJ; Luo D; Lee YJ; Harrison JK; Oh SP; Swanson MS, Developmental expression of mouse muscleblind genes Mbnl1, Mbnl2 and Mbnl3. Copyright 2003.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: Fl (forelimb bud), BI (first branchial arch), Nt (neural tube), Ov (otic vesicle), Oe (optic eminence). Sense probe failed to produce significant signal.
Expression Pattern Description
Spatial Annotation:
EMAGE:4130Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4130_wholemount_strong_3D_1.wlz
4130_wholemount_moderate_3D_1.wlz
4130_wholemount_weak_3D_1.wlz
4130_wholemount_possible_3D_1.wlz
4130_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4130_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
1st branchial arch mandibular component
strong strong
1st branchial arch maxillary component
strong strong
forelimb bud
moderate moderate
future brain
strong strong
eye
detected detected
regionalExpression is in the optic eminence.
otocyst
detected detected
nose
detected detected
regionalExpression is in the frontonasal prominence.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2683160
Entity Detected:Mbnl3, muscleblind-like 3 (Drosophila) ( MGI:2444912)
Notes:The Mbnl3 probe used in this study by Kanadia et al., 2003 [PMID:12915312] was isolated from an ES cell library and subcloned into pGEM-T Easy (Promega). To generate antisense RNA probes, the Mbnl3 [plasmid was] linearized with NcoI and transcribed with SP6 RNA polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Kanadia RN; Urbinati CR; Crusselle VJ; Luo D; Lee YJ; Harrison JK; Oh SP; Swanson MS, 2003 [PMID:12915312] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S1567-133X(03)00064-4] [ PMID:12915312] Kanadia RN, Urbinati CR, Crusselle VJ, Luo D, Lee YJ, Harrison JK, Oh SP, Swanson MS 2003 Developmental expression of mouse muscleblind genes Mbnl1, Mbnl2 and Mbnl3. Gene Expr Patterns (3):459-62
Links:MGI:2683243 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI