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EMAGE:4135

Kcnh6 potassium voltage-gated channel, subfamily H (eag-related), member 6 ( MGI:2684139)
TS16 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:4135
Figure 1B. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S1567-133X(03)00124-8] Gene Expr Patterns 3: 767-76, Polvani S; Masi A; Pillozzi S; Gragnani L; Crociani O; Olivotto M; Becchetti A; Wanke E; Arcangeli A, Developmentally regulated expression of the mouse homologues of the potassium channel encoding genes m-erg1, m-erg2 and m-erg3. Copyright 2003.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note. The authors state that there is specific signal in Encephalic vesicles (EV), spinal cord (SC) (see inset).
Expression Pattern Description
Spatial Annotation:
EMAGE:4135Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4135_wholemount_strong_3D_1.wlz
4135_wholemount_moderate_3D_1.wlz
4135_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4135_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
future brain
detected detected
Expression in encephalic vesicles (EV).
future spinal cord
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2684596
Entity Detected:Kcnh6, potassium voltage-gated channel, subfamily H (eag-related), member 6 ( MGI:2684139)
Sequence:sense strand is shown

>MGI:2684596
ACCTGGCCCAGCTCCTGGCCAAGAGCAGCAGCCGCAGCCTGACCCAACGTTTGTTGTCACACAGCTTCCT
GGGCTCTGAGGGCTCTCACAGCAGGCCAAGCGGACAGGGGCCTGGTCCAGGCAGGGGCAAGTATGGAACT
GTCAGCCAGATCCCGCAGTTCACGCTCAGCTTTGTGGAGTTCAACCTGGAGAAGCACCGCTCAGGCTCCA
CCACAGAGATTGAGATCATCGCTCCCCACAAGGTGGTGGAGCGGACACAGAATGTCACTGAAAAGGTCAC
GCAGGTCCTGTCCCTGGGTGCAGATGTGCTGCCAGAGTACAAGCTGCAGGCACCGCGCATTCACCGAGGT
ACCATTCTGCACTACAGCCCCTTCAAGGCTGTATGGGACTGGCTCATCTTGCTCTTAGTCATCTATACAG
CGGTCTTCACGCCCTACTCTGCTGCCTTTCTGCTTAGCGACCAGGACGACTCACAGCG
nt 392 - nt 869 of NM_001037712.1
Notes:The Kcnh6 (m-erg2) probe used in this study by Polvani et al., 2003 [PMID:14643686] was generated by RT-PCR using these primers which were designed against the rat sequence "m-erg2 (NM053937.1): sense: 5'-ACCTGGCCCAGCTCCTGG-3' (nucleotides 424-441); anti-sense: 5'-CGCGCTGTGATTCGTCCTG-3' (nucleotides 885-903)". Editors note: NM053937.1 is a typographical error and should appear as NM_053937.1. The co-ordinates given by the authors refer to the rat sequence NM_053937.1 - the corresponding position of the 'sense' primer with reference to the mouse cDNA Ref Seq NM_001037712.1 is nt 392-409, and the 'anti-sense' primer nt 853-869.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:9.5 dpc
Theiler Stage:TS16
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Polvani S, Masi A, Pillozzi S, Gragnani L, Crociani O, Olivotto M, Becchetti A, Wanke E, Arcangeli A, 2003 [PMID:14643686] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S1567-133X(03)00124-8] [ PMID:14643686] Polvani S, Masi A, Pillozzi S, Gragnani L, Crociani O, Olivotto M, Becchetti A, Wanke E, Arcangeli A 2003 Developmentally regulated expression of the mouse homologues of the potassium channel encoding genes m-erg1, m-erg2 and m-erg3. Gene Expr Patterns (3):767-76
Links:MGI:2684600 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI