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EMAGE:4146

Ndrg1 N-myc downstream regulated gene 1 ( MGI:1341799)
TS16 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4146
Figure 3A. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(99)00025-8] Mech Dev 83: 39-52, Shimono A; Okuda T; Kondoh H, N-myc-dependent repression of ndr1, a gene identified by direct subtraction of whole mouse embryo cDNAs between wild type and N-myc mutant. Copyright 1999.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: Somites (s), dorsal aorta (da, arrowhead). Scale bar: 1 mm. Authors state that staining of the brain and the dorsal aorta (da, arrowhead) was "non-specific".
Expression Pattern Description
Spatial Annotation:
EMAGE:4146Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4146_wholemount_strong_3D_1.wlz
4146_wholemount_moderate_3D_1.wlz
4146_wholemount_possible_3D_1.wlz
4146_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4146_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
trunk somite
weak weak
tail somite
weak weak
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3037912
Entity Detected:Ndrg1, N-myc downstream regulated gene 1 ( MGI:1341799)
Notes:The Ndrg1 (Ndr) probe used in this study by Shimono et al., 1999 [PMID:10381566] "was a 900 bp long EcoRI-HindIII fragment of cDNA". Editors Note: EcoRI does not cut the Ndrg1 mRNA Seq U60593.1 submitted by the authors to GenBank therefore is presumed to be linker derived. HindIII cuts once. Not enough information given to define probe sequence.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS16
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Shimono A; Okuda T; Kondoh H, 1999 [PMID:10381566] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S0925-4773(99)00025-8] [ PMID:10381566] Shimono A, Okuda T, Kondoh H 1999 N-myc-dependent repression of ndr1, a gene identified by direct subtraction of whole mouse embryo cDNAs between wild type and N-myc mutant. Mech Dev (83):39-52
Links:MGI:3038064 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI