EMAGE mouse gene expression spatial database

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EMAGE:4153

Sema4g sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4G ( MGI:1347047)
TS15 (9.5 dpc)
in situ hybridisation

Data Images


Figure 4C. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(99)00125-2] Mech Dev 87: 169-73, Li H; Wu DK; Sullivan SL, Characterization and expression of sema4g, a novel member of the semaphorin gene family. Copyright 1999.

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

Note: Scale bar: 500 microns. Ba, branchial acrh; Li, liver; Ta, tail.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

4153_wholemount_strong_3D_1.wlz

4153_wholemount_moderate_3D_1.wlz

4153_wholemount_possible_3D_1.wlz

4153_wholemount_notDetected_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:4153_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

visceral organ system

strong

regional

Expression is in the liver.

branchial arch

strong

regional

Expression is in the epithelial lining.

gut

strong

tail

strong

regional

Expression is in the most caudal region.

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	MGI:2674335
Entity Detected:	Sema4g, sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4G ( MGI:1347047)
Sequence:	sense strand is shown >MGI:2674335 AAGTGGACATCCTTCCTGAAGGCTCGTCTCATCTGCCACATCCCACAGTATGAGACACTACGTGGGGTCT GCAGCCTGAATGCTGATACATCATCCCACACACATTTCTATGCCGTCTTCACACTGACAACACAGTGGAA GACGCTGGAGGCTTCAGCCATCTGCCGCTATGACCTGGCTGAGATTCAGGCTGTCTTCACAGGCCCTTTT ATGGAGTACCAGGATGGTGCCCGGCGCTGGGGCCGCTATGAGGGTGGGGTGCCTGAACCCCGGCCTGGCT CGTGCATCACAGATTCATTGCGCAGCCGAGGCTACAACTCATCCCAAGACTTGCCATCCCTGGTTCTGGA CTTTGTGAAGTTGCATCCCCTGATGGCTCGGCCTGTGGTGCCCACACGTGGGCGGCCCTTGCTGCTGAAG CGCAATGTACGCTACACACATCTCACAGGAACACACGTCAGCACACCTGCTGGACCCACCTATGACCTGC TCTTTCTGGGCACAGCTGATGGCTGGATCCACAAGGCCGTAGTCCTGGGCTCTGGGATGCACATAATTGA AGAGATACAAGTGTTCAGGGAGCCCCAGTCTGTGGACAATCTAGTTATCTCACCAATGCAGCACAGCCTC TACGTGGGGGCTGCAAGTGGAGTCCTCCAGTTTCCTCTGTCCAGCTGCTCACGCTACCAGTCCTGCTATG ACTGCATCCTGGCACGGGACCCCTACTGTGGCTGGG
	nt 1232 - nt 1967 of NM_011976.1
Notes:	The Sema4g probe template used in this study by Li et al., 1999 [PMID:10495281] was generated as follows: "the 5' and 3' primers used were AARTGGACIACITTYYTIAARGC and TCCCAIGCRCARTRIGGRTC, respectively. A PCR product of ~750 bp encoding amino acids 280-525 of Sema4G was obtained". Editor's note: the nucleotide start and end co-ordinates of this fragment with respect to the Sema4g mRNA RefSeq NM_011976.1 were deduced from this information.
Chemistry:	RNA
Strand:	antisense

Specimen

Organism:	mouse
Age:	9.5 dpc
Theiler Stage:	TS15
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

General Information

Authors:	Li H; Wu DK; Sullivan SL, 1999 [PMID:10495281] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1016/S0925-4773(99)00125-2] [ PMID:10495281] Li H, Wu DK, Sullivan SL 1999 Characterization and expression of sema4g, a novel member of the semaphorin gene family. Mech Dev (87):169-73
Links:	MGI:2674365 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source