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EMAGE:4165

Dkk3 dickkopf homolog 3 (Xenopus laevis) ( MGI:1354952)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:4165
Figure 3C. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(99)00138-0] Mech Dev 87: 45-56, Monaghan AP; Kioschis P; Wu W; Zuniga A; Bock D; Poustka A; Delius H; Niehrs C, Dickkopf genes are co-ordinately expressed in mesodermal lineages. Copyright 1999.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: Arrow indicates neural epithelium.
Expression Pattern Description
Spatial Annotation:
EMAGE:4165Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4165_wholemount_strong_3D_1.wlz
4165_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4165_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
diencephalon
moderate moderate
regionalExpression is present in the diencephalon, either ventrally or medially.
future midbrain
weak weak
regionalExpression is present in the cranial flexure.
trunk mesenchyme
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3603151
Entity Detected:Dkk3, dickkopf homolog 3 (Xenopus laevis) ( MGI:1354952)
Sequence:sense strand is shown

>MGI:3603151
CTGATGGAAGACACTCAGCACAAACTGCGCAGTGCCGTGGAGGAGATGGAGGCGGAAGAAGCAGCTGCTA
AAACGTCCTCTGAGGTGAACCTGGCAAGCTTACCTCCCAACTATCACAATGAGACCAGCACGGAGACCAG
GGTGGGAAATAACACAGTCCATGTGCACCAGGAAGTTCACAAGATAACCAACAACCAGAGTGGACAGGTG
GTCTTTTCTGAGACAGTCATTACATCTGTAGGGGATGAAGAAGGCAAGAGGAGCCATGAATGTATCATTG
ATGAAGACTGTGGGCCCACCAGGTACTGCCAGTTCTCCAGCTTCAAGTACACCTGCCAGCCATGCCGGGA
CCAGCAGATGCTATGCACCCGAGACAGTGAGTGCTGTGGAGACCAGCTGTGT
nt 284 - nt 685 of NM_015814.2
Notes:The Dkk3 probe template used in this study by Monaghan et al., 1999 [PMID:10495270] was made as follows: "to clone mouse dkk-3 two primers were designed from two conserved regions showing high homology between human and chicken dkk-3 (f, CTGATGGAGGACACGCAGCAC. r, ACACAA/CAGCTGGTCTCC) and used to PCR-amplify a partial dkk-3 cDNA (0.4 kb) from new born mouse mRNA". Editors Note: the start and end co-ordinates of the probe with respect to the mouse mRNA RefSeq NM_015814.2 were deduced using this information.
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Strain:NMRI
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Monaghan AP; Kioschis P; Wu W; Zuniga A; Bock D; Poustka A; Delius H; Niehrs C, 1999 [PMID:10495270] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S0925-4773(99)00138-0] [ PMID:10495270] Monaghan AP, Kioschis P, Wu W, Zuniga A, Bock D, Poustka A, Delius H, Niehrs C 1999 Dickkopf genes are co-ordinately expressed in mesodermal lineages. Mech Dev (87):45-56
Links:MGI:3603172 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI