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EMAGE:417

Gli3 GLI-Kruppel family member GLI3 ( MGI:95729)
TS15 (28 Somite no.)
in situ hybridisation

Data Images
EMAGE:417
Figure 7A (Right) of Zuniga & Zeller, 1999 [PMID:9834182] . Copyright: This image is from Development and is displayed with permission of The Company of Biologists Ltd.

Expression pattern clarity: one star
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image is of a fore-limb bud. Asterisk denotes posterior limb-bud margin.
Expression Pattern Description
Spatial Annotation:
EMAGE:417Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
417_wholemount_strong_3D_1.wlz
417_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:417_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
forelimb bud
detected detected
regional
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1303143
Entity Detected:Gli3, GLI-Kruppel family member GLI3 ( MGI:95729)
Sequence:sense strand is shown

>MGI:1303143
GGTAACAAGCACAGTTGACAGCTTTGAGAGTCATGACCTAGAAGGTGTGCAGATTGATTTTGATGCCATC
ATAGATGATGGGGACCATACCAGCCTAATGTCAGGGGCCTTGAGCCCAAGTATTATTCAGAACCTTTCCC
ACAGCTCCTCCCGTCTCACCACTCCGCGGGCATCCCTCCCATTCCCAATCCCTATCCATGGGCACCACCA
ACATGGCTATCGGGGATATGAGTTCTTTGCTGACCTCCCTTGCAGAAGAAAGCAAGTTCCTTGCAGTTAT
GCAGTAGGCGGTAGGCAAGGAGGACCACAAACACAAAGACTGAAATGACTTGGGATTTTTTTTTCTTTTT
TAAGTTCTGTGTGTATTTTAGCAATCTCATCTCACCTGATTGGGATGTGTCTCAAGTATATTCCTTTTAT
GGAAAAGGACTCTGAAAAACCCTAAGGTATTCTAGGGGAGAAACTGTCTTCCATTTCAG
nt 4635 - nt 5113 of X95255.1
Notes:The probe used in this study by Zuniga & Zeller, 1999 [PMID:9834182] is indicated as that described by Buscher et al., 1997 [PMID:9152009] ie. a "cDNA fragment containing the 3' 478 bp of the murine Gli3 (Thien et al., 1996 [PMID:8688459] )".
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Strain:(C57BL/6 x SJL) F1
Age:28 Somite no.
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Zuniga & Zeller, 1999 [PMID:9834182] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:9834182] Zuniga A, Zeller R 1999 Gli3 (Xt) and formin (ld) participate in the positioning of the polarising region and control of posterior limb-bud identity. Development (126):13-21
 [ PMID:8688459] Thien H, Buscher D, Ruther U 1996 Cloning and sequence analysis of the murine Gli3 cDNA. Biochim Biophys Acta (1307):267-9
 [ doi:10.1016/S0925-4773(97)00656-4] [ PMID:9152009] Buscher D, Bosse B, Heymer J, Ruther U 1997 Evidence for genetic control of Sonic hedgehog by Gli3 in mouse limb development. Mech Dev (1997):175-82
Links:MGI:1314723 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE