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EMAGE:4171

Olfm1 olfactomedin 1 ( MGI:1860437)
TS16 (10 dpc)
in situ hybridisation

Data Images
EMAGE:4171
Figure 4H. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(02)00308-8] Mech Dev 119: 121, Moreno TA; Bronner-Fraser M, Neural expression of mouse Noelin-1/2 and comparison with other vertebrates. Copyright 2002.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: The tail is curved toward the viewer, causing the anterior/posterior axis of the limb to appear backwards. e, eye; hl, hindlimb; V, trigeminal ganglion; asterisk denotes otic vesicle.
Expression Pattern Description
Spatial Annotation:
EMAGE:4171Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4171_wholemount_strong_3D_1.wlz
4171_wholemount_moderate_3D_1.wlz
4171_wholemount_weak_3D_1.wlz
4171_wholemount_possible_3D_1.wlz
4171_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4171_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
future brain
detected detected
regionalExpression is in the marginal zones.
neural tube
detected detected
regionalExpression is in the marginal zones.
cranial ganglion
detected detected
dorsal root ganglion
detected detected
limb
detected detected
regional
trunk mesenchyme derived from neural crest
not detected not detected
homogeneousAbsent in migrating neural crest in the trunk.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2672938
Entity Detected:Olfm1, olfactomedin 1 ( MGI:1860437)
Sequence:sense strand is shown

>MGI:2672938
CAGAAGGCGATAACCGGGTCTGGTACATGGATGGTTATCACAACAACCGCTTCGTCCGTGAGTACAAGTC
CATGGTGGACTTCATGAACACGGACAACTTTACCTCTCACCGTCTCCCACACCCCTGGTCAGGTACGGGC
CAGGTGGTCTACAATGGCTCCATCTATTTCAACAAATTCCAGAGTCACATTATCATTAGGTTTGACCTGA
AGACAGAGACGATTCTGAAGACGCGCAGCCTGGACTATGCTGGCTACAACAATATGTATCACTATGCCTG
GGGCGGCCACTCAGACATCGACCTCATGGTGGACGAGAATGGGCTCTGGGCTGTCTATGCCACAAACCAG
AACGCAGGCAACATTGTCATCAGCAAGCTGGATCCTGTCTCACTGCAGATCCTCCAGACCTGGAACACCA
GCTACCCCAAGCGCAGTGCCGGTGAGGCCTTCATCATCTGCGGCACCCTCTATGTCACCAACGGTTACTC
GGGGGGCACCAAGGTCCACTATGCCTACCAAACCAACGCCTCGACCTACGAGTACATTGACATCCCCTTC
CAGAACAAATACTCTCACATCTCCATGCTGGACTACAACCCTAAAGACCGCGCGCTG
nt 1028 - nt 1644 of NM_019498.2
Notes:The Olfm1 (Noelin 1/2) probe template used in this study by Moreno & Bronner-Fraser, 2002 [PMID:12385760] "was generated using upstream primer 5'-CAGAAGGTGATAACCGG-3' and downstream primer 5'-CAGCGCGCGGTCTTTAG-3' to amplify a 616 base pair fragment of the Z exon from embryonic day 10 cDNA (Moreno & Bronner-Fraser, 2001 [PMID:11784068] )". Editors Note: the start and end co-ordinates of this fragment with respect to the mouse Olfm1 mRNA RefSeq NM_019498.2 were deduced using this information.
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Strain:C57BL/6J
Age:10 dpc
Theiler Stage:TS16
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Moreno TA & Bronner-Fraser M, 2002 [PMID:12385760] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S0925-4773(02)00308-8] [ PMID:12385760] Moreno TA, Bronner-Fraser M 2002 Neural expression of mouse Noelin-1/2 and comparison with other vertebrates. Mech Dev (119):121-5
 [ doi:10.1006/dbio.2001.0472] [ PMID:11784068] Moreno TA, Bronner-Fraser M 2001 The secreted glycoprotein Noelin-1 promotes neurogenesis in Xenopus. Dev Biol (240):340-60
Links:MGI:2672973 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI