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EMAGE:4176

Fgf20 fibroblast growth factor 20 ( MGI:1891346)
TS18 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4176
Figure 3C. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(01)00656-6] Mech Dev 113: 79-83, Hajihosseini MK; Heath JK, Expression patterns of fibroblast growth factors-18 and -20 in mouse embryos is suggestive of novel roles in calvarial and limb development. Copyright 2002.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4176Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4176_wholemount_strong_3D_1.wlz
4176_wholemount_moderate_3D_1.wlz
4176_wholemount_possible_3D_1.wlz
4176_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4176_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
limb
detected detected
regionalExpression is at the distal and peripheral regions.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2665355
Entity Detected:Fgf20, fibroblast growth factor 20 ( MGI:1891346)
Notes:The Fgf20 probe used in this study by Hajihosseini & Heath, 2002 [PMID:11900978] was either a "a 570 bp Fgf-20 riboprobe which would encompass regions of homology with Fgf-9" or "a 200 bp probe encoding the 5' nonhomologous region". See Table 1 therein for primer sequences relating to the latter probe.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL
Age:10.5 dpc
Theiler Stage:TS18
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Hajihosseini MK & Heath JK, 2002 [PMID:11900978] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S0925-4773(01)00656-6] [ PMID:11900978] Hajihosseini MK, Heath JK 2002 Expression patterns of fibroblast growth factors-18 and -20 in mouse embryos is suggestive of novel roles in calvarial and limb development. Mech Dev (113):79-83
Links:MGI:2665356 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI