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EMAGE:420

Pax6 paired box gene 6 ( MGI:97490)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:420
Figure 6A of Grindley et al., 1995 [PMID:7789273] . Copyright: This image is from Development and is displayed with permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Abbreviations used: ne, neuroepithelium of prosencephalon; npl, nasal placode.
Expression Pattern Description
Spatial Annotation:
EMAGE:420EMAGE:420Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context movie

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
420_voxel_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:420_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
lens placode
detected detected
Expression in nasal region. Authors report expression in nasal placode at a lower level than in lens placode.
olfactory placode
detected detected
Expression in nasal region. Authors report expression in nasal placode at a lower level than in lens placode.
nose
detected detected
Expression in nasal region. Authors report expression in nasal placode at a lower level than in lens placode.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:29788
Entity Detected:Pax6, paired box gene 6 ( MGI:97490)
Sequence:sense strand is shown

>MGI:29788
ATGGTTTTCTAATCGAAGGGCCAAATGGAGAAGAGAAGAGAAACTGAGGAACCAGAGAAGACAGGCCAGC
AACACTCCTAGTCACATACCTACCAGCAGCAGCTTCAGTACCAGTGTCTACCAGCCAATCCCACAGCCCA
CCACACCTGTCTCCTCCTTCACATCAGGTTCCATGTTGGGCCGAACAGACACCGCCCTCACCAACACGTA
CAGTGCTTTGCCACCCATGCCCAGCTTCACCATGGCAAACAACCTGCCTATGCAACCCCCAGTCCCCAGT
CAGACCTCCTCATACTCGTGCATGCTGCCCACCAGCCCGTCAGTGAATGGGCGGAGTTATGATACCTACA
CCCCTCCGCACATGCAAACACACATGAACAGTCAGCCCATGGGCACCTCGGGGACCACTTCAACAGGACT
CATTTCACCTGGAGTGTCAGTTCCCGTCCAAGTTCCCGGGAGTGAACCTGACATGTCTCAGTACTGGCCT
CGATAACGGTAAAGAGAGAAGGAGAGAGCATGTGATCGAGAGAGGAAATTGTGTTCACTCTGCCAATGAC
TATGTGGACACAGCAGTTGGGTATTCAGGAAAGAAAGAGAAATGGCGGTTAGAAGCACTTCACTTTGTAA
CTGTCCTGAACTGGAGCCCCGGAATGGACTAGAACCAAGGACCTTTGCGTACAGAAGGCACGGTATCAGT
TGGAACAAATCTTCATTTTGGTATCCAAACTTTTATTCATTTTGGTGTATTATTTGTAAATGGGCATTGG
TATGTTATTATGAAGAAAAGAACAACACAGGCTGTTGGATCGCGGATCTGTGTTGCTCATGTGGTTGTTT
AAAGGAAACCATGATCGACAAGATTTGCCATGGATTTAAGAGTTTTATCAAGATATATTAAATACTTCTC
CCCATCTGTTTATAGTTTATGGACTGATGTTCCAAGTTTGTATTATTCCTTTGGAAATAATTGAACCTGG
GACAACA
nt 638 - nt 1624 of M77842.1
Notes:The probe used in this study by Grindley et al., 1995 [PMID:7789273] is defined as a being transcribed from a "1 kb template", "made by PCR, from a 1.6 kb cDNA clone, pm1 (Ton et al., 1991 [PMID:1612585] ), using oligonucleotide C128 (ATGGTTTTCTAATCGAAGGG) from the 3' end of the Pax-6 homeobox, and a T7 promoter oligonucleotide (AATACGACTCAC-TATAG) matching vector sequence".
Chemistry:RNA
Strand:antisense
Label:S35
Specimen
Organism:mouse
Strain:Swiss
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Staining procedure:autoradiography
General Information
Authors:Grindley et al., 1995 [PMID:7789273] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:7789273] Grindley JC, Davidson DR, Hill RE 1995 The role of Pax-6 in eye and nasal development. Development (121):1433-42
 [ doi:10.1016/0092-8674(91)90284-6] [ PMID:1684738] Ton CC, Hirvonen H, Miwa H, Weil MM, Monaghan P, Jordan T, van Heyningen V, Hastie ND, Meijers-Heijboer H, Drechsler M 1991 Positional cloning and characterization of a paired box- and homeobox-containing gene from the aniridia region. Cell (67):1059-74
Links:MGI:2385724 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE