EMAGE mouse gene expression spatial database

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EMAGE:4221

Nrarp Notch-regulated ankyrin repeat protein ( MGI:1914372)
TS15 (9.5 dpc)
in situ hybridisation

Data Images


Fig4B. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.2001.0408] Dev Biol 238: 110-9, Krebs LT; Deftos ML; Bevan MJ; Gridley T, The nrarp gene encodes an ankyrin-repeat protein that is transcriptionally regulated by the notch signaling pathway. Copyright 2001	Fig4C. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.2001.0408] Dev Biol 238: 110-9, Krebs LT; Deftos ML; Bevan MJ; Gridley T, The nrarp gene encodes an ankyrin-repeat protein that is transcriptionally regulated by the notch signaling pathway. Copyright 2001

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

Fig4C is a higher magnification of the embryo shown in Fig4B, with the midbrain region indicated by the white bracket.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

4221_wholemount_strong_3D_1.wlz

4221_wholemount_moderate_3D_1.wlz

4221_wholemount_weak_3D_1.wlz

4221_wholemount_notDetected_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:4221_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

future forebrain

strong

future hindbrain

strong

future spinal cord

strong

central nervous system ganglion

strong

future midbrain

not detected

homogeneous

trunk paraxial mesenchyme

strong

regional

There is an anterior stripe and a more extensive posterior domain.

tail paraxial mesenchyme

strong

regional

There is an anterior stripe and a more extensive posterior domain.

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	MGI:2153517
Entity Detected:	Nrarp, Notch-regulated ankyrin repeat protein ( MGI:1914372)
Notes:	The Nrarp probe used in this study by Krebs et al., 2001 [PMID:11783997] is described as follows: "The Nrarp in situ probe consisted of a 1.1-kb EcoRI fragment, which contained 300 nucleotides encoding the carboxy-terminus of the protein and 800 nucleotides of the 3' untranslated region." Editor's note: The authors provide the GenBank ID for the sequence of Nrarp (AY046077.1), which is a composite of the sequence from the clone discussed above and sequence obtained from several EST reads. EcoRI cuts AY046077.1 only once (within the ORF and 224 nt from the stop codon). Ambiguous information prevents defining the exact probe sequence based on published information
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	9.5 dpc
Theiler Stage:	TS15
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

Fixation:	4% paraformaldehyde
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Krebs LT; Deftos ML; Bevan MJ; Gridley T, 2001 [PMID:11783997] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1006/dbio.2001.0408] [ PMID:11783997] Krebs LT, Deftos ML, Bevan MJ, Gridley T 2001 The Nrarp gene encodes an ankyrin-repeat protein that is transcriptionally regulated by the notch signaling pathway. Dev Biol (238):110-9
Links:	MGI:2154092 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source