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EMAGE:4231

Aatf apoptosis antagonizing transcription factor ( MGI:1929608)
TS19 (11.5 dpc)
in situ reporter

Data Images
EMAGE:4231
Fig4B. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.2000.9915] Dev Biol 227: 324-42, Thomas T; Voss AK; Petrou P; Gruss P, The murine gene, traube, is essential for the growth of preimplantation embryos Copyright 2000

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4231Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4231_wholemount_strong_3D_1.wlz
4231_wholemount_moderate_3D_1.wlz
4231_wholemount_weak_3D_1.wlz
4231_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4231_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
brain
strong strong
regionalExpression was detected in the posterior commissure.
embryo
weak weak
Expression was low in many tissues.
hindbrain
strong strong
future spinal cord
strong strong
dorsal root ganglion
strong strong
liver
strong strong
Expression of Trb (Aatf) in the liver was restricted to the hepatocytes.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ reporter
Entity Detected:Aatf, apoptosis antagonizing transcription factor ( MGI:1929608)
Notes:Aatf (Trb) expression was detected in this study by Thomas et al., 2000 [PMID:11071758] as follows: The promoterless reporter construct pGT1.8geo (kindly provided by W. Skarnes; Skarnes et al., 1995 [PMID:7604039] ) was electroporated into the parental murine ES cell line MPI-II (Voss et al., 1997 [PMID:9013705] ) as described previously (Voss et al., 1998 [PMID:9626493] ). Chimeras were produced by a technique modified from Nagy and Rossant (1993) omitting the sandwich technique (Voss et al., 1998 [PMID:9626493] ). Chimeras were mated to wild-type females of the mouse strains C57Bl/6 and NMRI to produce animals heterozygous for the Trb<gt> mutation.
Labelled with:LacZ
Specimen
Organism:mouse
Strain:either: (129/Sv * C57BL/6) or (129/Sv * NMRI)
Age:11.5 dpc
Theiler Stage:TS19
Mutations:AatfGt(pGT1.8geo)3Pgr/Aatf+
Preparation:wholemount
Procedures
General Information
Authors:Thomas T; Voss AK; Petrou P; Gruss P, 2000 [PMID:11071758] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1006/dbio.2000.9915] [ PMID:11071758] Thomas T, Voss AK, Petrou P, Gruss P 2000 The murine gene, Traube, is essential for the growth of preimplantation embryos. Dev Biol (227):324-42
 [ doi:10.1073/pnas.92.14.6592] [ PMID:7604039] Skarnes WC, Moss JE, Hurtley SM, Beddington RS 1995 Capturing genes encoding membrane and secreted proteins important for mouse development. Proc Natl Acad Sci U S A (92):6592-6
 [ doi:10.1006/excr.1996.3418] [ PMID:9013705] Voss AK, Thomas T, Gruss P 1997 Germ line chimeras from female ES cells. Exp Cell Res (230):45-9
 [ doi:10.1002/(SICI)1097-0177(199806)212:2<171::AID-AJA3>3.0.CO;2-E] [ PMID:9626493] Voss AK, Thomas T, Gruss P 1998 Efficiency assessment of the gene trap approach. Dev Dyn (212):171-80
Links:MGI:3036523 same experiment
 MGI:2176283 allele description
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI