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EMAGE:4255

Spint1 serine protease inhibitor, Kunitz type 1 ( MGI:1338033)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:4255
Supplementary Fig5-Spint1(right). Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.ydbio.2007.01.011] Dev Biol 304: 541-55, Sherwood RI; Jitianu C; Cleaver O; Shaywitz DA; Lamenzo JO; Chen AE; Golub TR; Melton DA, Prospective isolation and global gene expression analysis of definitive and visceral endoderm. Copyright 2007

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4255Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4255_wholemount_strong_3D_1.wlz
4255_wholemount_moderate_3D_1.wlz
4255_wholemount_weak_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4255_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
embryo
strong strong
regionalExpression was strong not only in endodermal regions but in other germ layers.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3718625
Entity Detected:Spint1, serine protease inhibitor, Kunitz type 1 ( MGI:1338033)
Notes:The Spint1 probe used in this study by Sherwood et al., 2007 [PMID:17328885] was obtained from a commercial clone library (Open Biosystems, Huntsville, AL), yet is of is of unreported sequence
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:ICR
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Sherwood RI; Jitianu C; Cleaver O; Shaywitz DA; Lamenzo JO; Chen AE; Golub TR; Melton DA, 2007 [PMID:17328885] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1016/j.ydbio.2007.01.011] [ PMID:17328885] Sherwood RI, Jitianu C, Cleaver O, Shaywitz DA, Lamenzo JO, Chen AE, Golub TR, Melton DA 2007 Prospective isolation and global gene expression analysis of definitive and visceral endoderm. Dev Biol (304):541-55
Links:MGI:3718659 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI