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EMAGE:4266

Gcm2 glial cells missing homolog 2 (Drosophila) ( MGI:1861438)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4266
Fig3A. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.ydbio.2007.02.014] Dev Biol 305: 333-46, Liu Z; Yu S; Manley NR, Gcm2 is required for the differentiation and survival of parathyroid precursor cells in the parathyroid/thymus primordia. Copyright 2007

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: arrow indicates dorsal/anterior parathyroid domain.
Expression Pattern Description
Spatial Annotation:
EMAGE:4266Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4266_wholemount_strong_3D_1.wlz
4266_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4266_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
3rd branchial pouch dorsal endoderm
detected detected
regionalExpression was detected in a small dorso-anterior domain of the third pouch (presumptive parathyroid domain).
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3722625
Entity Detected:Gcm2, glial cells missing homolog 2 (Drosophila) ( MGI:1861438)
Sequence:sense strand is shown

>MGI:3722625
GCAAGAAGCACTCAGGACATCAGGGGACACCTCAATAGCACGGCTGCCCTGGAACCCACAGAACTATTTG
ATATGACTGCTGATACCAGCTTCCCTATTCCAGGGCAGCCCTCCCCTTCGTTCCCAAACTCTGATGTCTA
CAGAGTTACCTGTGACCTGCCCACCTTTCAAGGAGATATAATACTGCCCTTTCAGAAATATCCAAATCCA
AGCATTTATTTCCCCGGGCCACCTTGGGGCTATGAGTTGGCAAGTTCTGGGGTTACAGGTTCGAGTCCAT
ATTCCACCCTGTATAAAGATTCCTCCGAGGTCCCCGATGACCCAGATTGGATTCCTCTAAACTCACTACA
GTATAATGTCAGTTCCTATGGCAGCTATGAGAGAACCTTGGATTTCACAGCTAGGTATCATAGCTGGAAA
CCAACACACGGGAAGCCCAGCCTTGAGGAGAAGGTTGACTGTGAGCAATGCCAGGCTGTGCCCACATTGC
CTTATTACAACCTAGAGCTGCCCTGCAGGTACCTGCCAGTGCCAGCAGCGGGTAGCCAGGCTCTGCAGAC
AGTAATCACCACCACAGTGGCTTACCAGGCCTACCAGCACCCTGCTCTGAAACACGGTGACAGCATGCAG
GAGGTTAGCAGCCTTGCCAGCTGCACCTATGCCTCGGAAAACCTCCCAATGCCCATCTATCCACCAGCCT
TAGACCCTCAAGAGGGAGTCATTCAGGCAGTCTCTCCTTCAGGGAGAGCCCCTCTGAAAGTCCCTGGAGA
CTGCCAGGCCCCCAGACCCACTCTGGATTTTCCTCAAGAAGCAGATCCCTCCGGGACAGATGGAGCAGAT
GTGTGGGATGTGTGTCTGTCTGGGGTGGGCTCTGTGATGGGTTACTTAGATAGGACAGGGCAGCCCTTTA
GCTTTGACAATGAGGACT
nt 617 - nt 1544 of NM_008104.2
Notes:The Gcm2 probe used in this study by Liu et al., 2007 [PMID:17382312] was previously used by Gordon et al., 2001 [PMID:11335122] and Kim et al., 1998 [PMID:9770492] who describes it as follows: "For the mGcm2 probe template, a pair of primers (5'-ATGCGAATTCGCAAGAAGCACTCAGGAC-3' and 5'-CTAGTCTAGAGTCCTCATTGTCAAAGCTAAAGGGC-3') was used to PCR amplify a 927-base fragment corresponding to the 3' end exon of mGcm2 from mouse genomic DNA. After EcoRI and XbaI digestion, the fragment was inserted into the pBluescript KS (+) plasmid. " Editors note: The primers contain the EcoRI/XbaI sequences.
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Liu Z; Yu S; Manley NR, 2007 [PMID:17382312] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/j.ydbio.2007.02.014] [ PMID:17382312] Liu Z, Yu S, Manley NR 2007 Gcm2 is required for the differentiation and survival of parathyroid precursor cells in the parathyroid/thymus primordia. Dev Biol (305):333-46
 [ doi:10.1016/S0925-4773(01)00333-1] [ PMID:11335122] Gordon J, Bennett AR, Blackburn CC, Manley NR 2001 Gcm2 and Foxn1 mark early parathyroid- and thymus-specific domains in the developing third pharyngeal pouch. Mech Dev (103):141-3
 [ doi:10.1073/pnas.95.21.12364] [ PMID:9770492] Kim J, Jones BW, Zock C, Chen Z, Wang H, Goodman CS, Anderson DJ 1998 Isolation and characterization of mammalian homologs of the Drosophila gene glial cells missing. Proc Natl Acad Sci U S A (95):12364-9
Links:MGI:3722792 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI