Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:4268

Espn espin ( MGI:1861630)
TS17 (10.5 dpc)
immunohistochemistry

Data Images
EMAGE:4268
Fig1K. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.ydbio.2005.12.021] Dev Biol 291: 83-95, Sekerkova G; Zheng L; Mugnaini E; Bartles JR, Differential expression of espin isoforms during epithelial morphogenesis, stereociliogenesis and postnatal maturation in the developing inner ear. Copyright 2006

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: Asterisk - otocyst. I, II - branchial arches.
Expression Pattern Description
Spatial Annotation:
EMAGE:4268Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4268_wholemount_strong_3D_1.wlz
4268_wholemount_possible_3D_1.wlz
4268_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4268_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
inner ear
detected detected
Expression is present in the otic pit, the otocyst, the developing membranous labyrinth (including endoymphatic duct, vertical canal pouch, anterior and posterior semicircular canal), and the clefts between branchial arches.
otocyst
detected detected
Expression is present in the otic pit, the otocyst, the developing membranous labyrinth (including endoymphatic duct, vertical canal pouch, anterior and posterior semicircular canal), and the clefts between branchial arches.
endolymphatic appendage
detected detected
Expression is present in the otic pit, the otocyst, the developing membranous labyrinth (including endoymphatic duct, vertical canal pouch, anterior and posterior semicircular canal), and the clefts between branchial arches.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:MGI:3623765
Entity Detected:Espn, espin ( MGI:1861630)
Notes:The antibody used in this study by Sekerkova et al., 2006 [PMID:16413524] is described as affinity purified rabbit pan-espin antibody. "The epsin antibody, which was raised against purified recombinant rat espin 2B and affinity purified on columns of rat espin 2B-Sepharose 4B, is known to react with all espin isoforms."
Antibody Type:polyclonal
Raised In:rabbit
Specimen
Organism:mouse
Strain:involves: fancier's dancing mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:Espnje/Espn+
Preparation:wholemount
Procedures
Fixation:2% paraformaldehyde
Secondary Antibody:biotinylated donkey anti-rabbit IgG
Tertiary Antibody:streptavidin-HRP
Labelled with:horse radish peroxidase
Visualisation method:DAB
General Information
Authors:Sekerkova G; Zheng L; Mugnaini E; Bartles JR, 2006 [PMID:16413524] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/j.ydbio.2005.12.021] [ PMID:16413524] Sekerkova G, Zheng L, Mugnaini E, Bartles JR 2006 Differential expression of espin isoforms during epithelial morphogenesis, stereociliogenesis and postnatal maturation in the developing inner ear. Dev Biol (291):83-95
Links:MGI:3623768 same experiment
 MGI:1856573 allele description
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI