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EMAGE:4274

Flrt3 fibronectin leucine rich transmembrane protein 3 ( MGI:1918686)
TS16 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4274 EMAGE:4274 EMAGE:4274 EMAGE:4274
Fig2D. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.ydbio.2006.04.004] Dev Biol 297: 14-25, Haines BP; Wheldon LM; Summerbell D; Heath JK; Rigby PW, Regulated expression of FLRT genes implies a functional role in the regulation of FGF signalling during mouse development. Copyright 2006 Fig2Di. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.ydbio.2006.04.004] Dev Biol 297: 14-25, Haines BP; Wheldon LM; Summerbell D; Heath JK; Rigby PW, Regulated expression of FLRT genes implies a functional role in the regulation of FGF signalling during mouse development. Copyright 2006 Fig2Dii. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.ydbio.2006.04.004] Dev Biol 297: 14-25, Haines BP; Wheldon LM; Summerbell D; Heath JK; Rigby PW, Regulated expression of FLRT genes implies a functional role in the regulation of FGF signalling during mouse development. Copyright 2006 Fig2Diii. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.ydbio.2006.04.004] Dev Biol 297: 14-25, Haines BP; Wheldon LM; Summerbell D; Heath JK; Rigby PW, Regulated expression of FLRT genes implies a functional role in the regulation of FGF signalling during mouse development. Copyright 2006

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: i-iii are sections of the embryo in D with the approximate planes of section shown by yellow lines. s-somites; vfb-hypothalamic region of the ventral forebrain; fmb-forebrain/midbrain boundary; aer-apical ectodermal ridge; hm-head mesoderm.
Expression Pattern Description
Spatial Annotation:
EMAGE:4274Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4274_wholemount_strong_3D_1.wlz
4274_wholemount_moderate_3D_1.wlz
4274_wholemount_weak_3D_1.wlz
4274_wholemount_possible_3D_1.wlz
4274_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4274_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
future forebrain
detected detected
regionalExpression is in the midbrain at the boundaries with the hindbrain and forebrain
future midbrain
detected detected
regionalExpression is in the midbrain at the boundaries with the hindbrain and forebrain
future hindbrain
detected detected
regionalExpression is in the midbrain at the boundaries with the hindbrain and forebrain
forelimb bud apical ectodermal ridge
detected detected
trunk mesenchyme
detected detected
regionalExpression is in the migrating epithelial structure surrounding the lower region of the developing heart
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3689565
Entity Detected:Flrt3, fibronectin leucine rich transmembrane protein 3 ( MGI:1918686)
Sequence:sense strand is shown

>MGI:3689565
GAGACAGTAACTATCTGCGGCTGCTTTTTCTGTCCCGTAACCACCTTAGCACAATCCCGGGGGGCTTGCC
CAGGACTATTGAGGAATTACGCCTGGATGACAATCGCATATCAACGATCTCTTCCCCATCACTTCATGGT
CTCACAAGCCTGAAACGCCTGGTTTTAGATGGAAACTTGTTGAACAACCATGGTCTGGGTGACAAAGTTT
TCTTCAACTTAGTAAACTTAACAGAGCTGTCCCTGGTGAGGAATTCCTTGACAGCAGCGCCAGTGAACCT
TCCCGGCACAAGCCTGAGGAAGCTTTACCTTCAAGACAACCATATCAACCGGGTACCCCCAAATGCTTTT
TCTTATTTAAGGCAGCTGTATCGACTCGATATGTCTAATAATAACCTAAGCAATTTACCTCAGGGTATCT
TTGATGATTTGGACAATATAACCCAACTGATTCTTCGCAACAATCCTTGGTATTGTGGATGCAAGATGAA
ATGGGTACGAGACTGGTTACAGTCGCTA
nt 1 - nt 518 of BG922680.1
Notes:The Flrt3 probe used in this study by Haines et al., 2006 [PMID:16872596] is described as "transcribed using T3 polymerase from a BamHI digest of the plasmid FLRT3 Age, which was made by digesting the FLRT3 image clone 4950755 with AgeI and cloning the 5' most fragment into XmaI cut pBluescript II KS." Editors Note: The most 5' AgeI cut site within the insert sequence of IMAGE:4950755 is at position 518 of BG922680.1.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS16
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Staining procedure:alkaline phosphatase + NBT/Red Phos
General Information
Authors:Haines BP; Wheldon LM; Summerbell D; Heath JK; Rigby PW, 2006 [PMID:16872596] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/j.ydbio.2006.04.004] [ PMID:16872596] Haines BP, Wheldon LM, Summerbell D, Heath JK, Rigby PW 2006 Regulated expression of FLRT genes implies a functional role in the regulation of FGF signalling during mouse development. Dev Biol (297):14-25
Links:MGI:3689513 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI