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EMAGE:4284

Flrt2 fibronectin leucine rich transmembrane protein 2 ( MGI:3603594)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4284 EMAGE:4284 EMAGE:4284 EMAGE:4284 EMAGE:4284
Fig5B. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.ydbio.2006.04.004] Dev Biol 297: 14-25, Haines BP; Wheldon LM; Summerbell D; Heath JK; Rigby PW, Regulated expression of FLRT genes implies a functional role in the regulation of FGF signalling during mouse development. Copyright 2006 Fig5Bi. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.ydbio.2006.04.004] Dev Biol 297: 14-25, Haines BP; Wheldon LM; Summerbell D; Heath JK; Rigby PW, Regulated expression of FLRT genes implies a functional role in the regulation of FGF signalling during mouse development. Copyright 2006 Fig5Bii. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.ydbio.2006.04.004] Dev Biol 297: 14-25, Haines BP; Wheldon LM; Summerbell D; Heath JK; Rigby PW, Regulated expression of FLRT genes implies a functional role in the regulation of FGF signalling during mouse development. Copyright 2006 Fig5Biii. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.ydbio.2006.04.004] Dev Biol 297: 14-25, Haines BP; Wheldon LM; Summerbell D; Heath JK; Rigby PW, Regulated expression of FLRT genes implies a functional role in the regulation of FGF signalling during mouse development. Copyright 2006 Fig5Biv. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.ydbio.2006.04.004] Dev Biol 297: 14-25, Haines BP; Wheldon LM; Summerbell D; Heath JK; Rigby PW, Regulated expression of FLRT genes implies a functional role in the regulation of FGF signalling during mouse development. Copyright 2006
EMAGE:4284
Fig5Bv. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.ydbio.2006.04.004] Dev Biol 297: 14-25, Haines BP; Wheldon LM; Summerbell D; Heath JK; Rigby PW, Regulated expression of FLRT genes implies a functional role in the regulation of FGF signalling during mouse development. Copyright 2006

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: i-v are sections of the embryo in Fig5B with the approximate planes of section shown by yellow lines. The anterior (A) side of section v is shown. cm - cephalic mesenchyme; tm - trunk mesenchyme; scl - sclerotome; dm - dermomyotome, nt - neural tube; bw - body wall; drg - dorsal root ganglia; st - stomach.
Expression Pattern Description
Spatial Annotation:
EMAGE:4284Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4284_wholemount_strong_3D_1.wlz
4284_wholemount_moderate_3D_1.wlz
4284_wholemount_weak_3D_1.wlz
4284_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4284_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
head mesenchyme
strong strong
regionalExpression is in the ventral mesoderm adjacent to the forebrain. Expression is in cephalic mesenchyme adjacent to the ventral neural vesicle.
trunk mesenchyme
strong strong
regionalExpression is in segmental stripes. Expression is in mesenchymal tissue in the anterior trunk that crosses the embryo ventral to the neural tube. Expression is in a group of cells that lie between the dorsal root ganglia and the dermomyotomes of the somites.
body-wall mesenchyme
strong strong
regionalExpression is posterior to the developing heart in the body wall overlying the pericardial cavity.
stomach
strong strong
trunk somite
detected detected
regionalExpression is in a subset of the sclerotome.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3689561
Entity Detected:Flrt2, fibronectin leucine rich transmembrane protein 2 ( MGI:3603594)
Sequence:sense strand is shown

>MGI:3689561
AACTGACATGGGTGAAAATGGGCCACAGTCTCGTAGGGGGCATCGTTCAGGAACGAATTGTCAGTGGTGA
GAAGCAACACCTGAGCTTGGTTAATTTAGAGCCCAGATCCACGTATAGGATTTGTTTAGTGCCGCTGGAT
GCGTTCAACTACCGCACTGTGGAAGATACCATCTGTTCGGAGGCTACCACCCATGCCTCTTATTTGAACA
ACGGCAGCAACACTGCTTCTAGCCATGAGCAGACGACTTCCCACAGTATGGGCTCCCCTTTTCTGCTCGC
AGGCTTGATTGGGGGCGCAGTGATTTTTGTGCTCGTTGTCTTGCTCAGCGTCTTTTGCTGGCACATGCAC
AAAAAGGGACGCTACACCTCCCAGAAGTGGAAATACAACCGGGGCCGACGGAAAGACGACTATTGTGAAG
CGGGTACCAAAAAAGACAACTCCATCTTGGAGATGACAGAAACAAGTTTTCAGATTGTCTCCTTAAATAA
CGATCAGCTCCTTAAAGGAGATTTCAGACTGCA
nt 2097 - nt 2619 of NM_201518.2
Notes:The Flrt2 probe used in this study by Haines et al., 2006 [PMID:16872596] is described as follows: "A riboprobe for FLRT2 was transcribed using T3 polymerase from a BamHI digest of the plasmid FLRT2 Pst, which was made by digesting the FLRT2 image clone 553778 with PstI and cloning the 5' most fragment into PstI cut pBluescript II KS (Stratagene)". Editors Note: the start and end points of this probe template were deduced as such: the 5' sequence read of IMAGE:553778 (AA098484.1) was aligned to the mouse Flrt2 mRNA RefSeq NM_201518.2 indicating that the 5' end of the insert of IMAGE:553778 is found at nt2097 in NM_201518.2. Subsequent restriction digestion of NM_201518.2 indicates that PstI cuts at one site 3' to nt2097 (i.e. at nt2619). PstI also cuts IMAGE:553778 in the polylinker adjacent to the SalI cloning site at the 5' end of the insert, therefore the PstI fragment cloned into pBluescript II KS to create FLRT2 Pst is most likely to have spanned nt2097-2619 in NM_201518.2.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Staining procedure:alkaline phosphatase + NBT/Red Phos
General Information
Authors:Haines BP; Wheldon LM; Summerbell D; Heath JK; Rigby PW, 2006 [PMID:16872596] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/j.ydbio.2006.04.004] [ PMID:16872596] Haines BP, Wheldon LM, Summerbell D, Heath JK, Rigby PW 2006 Regulated expression of FLRT genes implies a functional role in the regulation of FGF signalling during mouse development. Dev Biol (297):14-25
Links:MGI:3689562 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI