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EMAGE:4292

Otx2 orthodenticle homolog 2 (Drosophila) ( MGI:97451)
TS19 (11.5 dpc)
in situ hybridisation

Data Images
EMAGE:4292 EMAGE:4292 EMAGE:4292
OTX2_E11.5_lls OTX2_E11.5_front OTX2_E11.5_rls

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4292Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4292_wholemount_strong_3D_1.wlz
4292_wholemount_moderate_3D_1.wlz
4292_wholemount_weak_3D_1.wlz
4292_wholemount_possible_3D_1.wlz
4292_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4292_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Otx2_T7
Entity Detected:Otx2, orthodenticle homolog 2 (Drosophila) ( MGI:97451)
Sequence:sense strand is shown

>Otx2_T7
CTGCCAGGCTTACCAACATTAAATTACGGGTGAATTAGGGTCCTTTTGCTTCTTCTCGATCCTTGTCCAG
AGACAGCAGGGCAGCTCACCTCCAGCCCAAGGTAATCTTTCTAGATCAGTATCCAGTTGCTCTGAATTTT
GCTTATAATTATAACCTATTTAATCACAGAAGAACCCCTGCGGAGGTGGAGTTCAAGGTTGCATACAATA
ACAGGAGATCACAGTTTTGAAGTCTAGCACAGATTAAAAACCACAGATGTACCATTTATATAAGACACAC
ACTGATTGTCTCTTAAACTACATATTGACTCCGTATGAGCGGTATCTTTTTTTTTTTTTTTAAATAAAGT
AGTGCATCTAGGACAATCAGTCGCACAATCCACACAGCCCTGAAAAGTTTTTCAGTGCCAACTACCTGTT
GGTCATGAAACGTGAATGAGCCTGGGACATTGTCCATTCCTAAGATTCAACCAAAGATTGGCTAAAACAC
CGGATCACCTCTG
nt 47580298 - nt 47579796 of NC_000080.4
Notes:These primers were used to generate the in vitro transcription template by PCR of mouse whole genomic DNA. They contain T7 or T3 promotor sequences and amplify a region corresponding to a portion of the 3'UTR. T3 forward primer (sense) 5'-3' AATTAACCCTCACTAAAGGCAGAGGTGATCCGGTGTTTT T7 reverse primer (antisense) 5'-3' TAATACGACTCACTATAGGCTGCCAGGCTTACCAACATT Product size: 503bp
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD-1
Age:11.5 dpc
Theiler Stage:TS19
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Jacqueline Ramsay, David R FitzPatrick
Principal investigator:David R FitzPatrick, Medical Research Council, Human Genetics Unit MRC Western General Hospital Crewe Road, Edinburgh, Scotland EH4 2XU
Submitted by:Jacqueline Ramsay, Medical Research Council, Human Genetics Unit MRC Western General Hospital Crewe Road, Edinburgh, Scotland EH4 2XU
Experiment type:screen
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceFACEBASE