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EMAGE:4295

Six3 sine oculis-related homeobox 3 homolog (Drosophila) ( MGI:102764)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:4295
Fig4E. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.2001.0531] Dev Biol 242: 204-23, Tian E; Kimura C; Takeda N; Aizawa S; Matsuo I, Otx2 is required to respond to signals from anterior neural ridge for forebrain specification. Copyright 2002

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4295Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4295_wholemount_strong_3D_1.wlz
4295_wholemount_moderate_3D_1.wlz
4295_wholemount_possible_3D_1.wlz
4295_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4295_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
future forebrain
detected detected
regional
eye
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1315062
Entity Detected:Six3, sine oculis-related homeobox 3 homolog (Drosophila) ( MGI:102764)
Notes:The Six3 probe used in this study by Tian et al, 2002 [PMID:11820816] is indicated as that used by Oliver et al., 1995 [PMID:8575305] , which is of unreported sequence.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:involves: C57BL/6 * CBA
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Tian E; Kimura C; Takeda N; Aizawa S; Matsuo I, 2002 [PMID:11820816] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1006/dbio.2001.0531] [ PMID:11820816] Tian E, Kimura C, Takeda N, Aizawa S, Matsuo I 200 Otx2 is required to respond to signals from anterior neural ridge for forebrain specification. Dev Biol (242):204-23
Links:MGI:2179003 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI