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EMAGE:4296

Tgfa transforming growth factor alpha ( MGI:98724)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4296 EMAGE:4296
TFGA_e10.5_rls TFGA_e10.5_lls

Expression pattern clarity: one star
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
The apparent signal in the head may be an artifact due to reagent trapping.
Expression Pattern Description
Spatial Annotation:
EMAGE:4296Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4296_wholemount_possible_3D_1.wlz
4296_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4296_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
embryo
possible possible
regional
Annotation Validation: Submitter + EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:TgfAlpha_T7
Entity Detected:Tgfa, transforming growth factor alpha ( MGI:98724)
Sequence:sense strand is shown

>TgfAlpha_T7
TGGGTCATACAAGTGGGTCAAGGCCAAGTGTCACCTGCTGCTCAGACACCAATCAACCTCACTGGACTTC
AGCCCTCTATTGGCCCCCAGTGCTCTCAGTAGACCCCGCCTGAAGTACTGGCTCACTGGAGGGCAGGGAG
CAACACAAATGGAGGAGATGTGGGGCTTGTTTTAGTTTAGTTCACACTGTTAGCTTCTGATGACAGGTCT
TTTCTGGTCTGCTGGAGGCTCCTGGGTGTCTGACTTCTTTTAAAGAGTTTGAACTGATATCAAAATATCA
TTGGGAACTTCCTCACTTAATATCCCATCCTGAAGATGTTGTCATCCAAGACTAAGCAGAGGTGGTAAAC
GCTTAGCACGAGCACTCGATGGTGAAGGCAGAGGATCTCGGGAGGACCTCAGCTTTAGTGTCCAAACGGT
CCTGTTCTCAGGTCCAGCCAGTCGCAGCAGCCAGTACCATCCCAGTGCCTGTACACAGATGCAGCTGGCT
CAAACACCTCCCAGAAG
nt 86239279 - nt 86239785 of NC_000072.4
Notes:These primers were used to generate the in vitro transcription template by PCR of mouse whole genomic DNA. They contain T7 or T3 promotor sequences. T3 forward primer (sense) 5'-3' AATTAACCCTCACTAAAGGTGGGTCATACAAGTGGGTCA T7 reverse primer (antisense) 5'-3' TAATACGACTCACTATAGGCTTCTGGGAGGTGTTTGAGC Product size:507
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD-1
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Jacqueline Ramsay, David R FitzPatrick
Principal investigator:David R FitzPatrick, Medical Research Council, Human Genetics Unit MRC Western General Hospital Crewe Road, Edinburgh, Scotland EH4 2XU
Submitted by:Jacqueline Ramsay, Medical Research Council, Human Genetics Unit MRC Western General Hospital Crewe Road, Edinburgh, Scotland EH4 2XU
Experiment type:screen
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceFACEBASE