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EMAGE:4299

Dlx3 distal-less homeobox 3 ( MGI:94903)
TS19 (11.5 dpc)
in situ hybridisation

Data Images
EMAGE:4299 EMAGE:4299

Expression pattern clarity: one star
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4299Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4299_wholemount_strong_3D_1.wlz
4299_wholemount_possible_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4299_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:DLX3_probeA
Entity Detected:Dlx3, distal-less homeobox 3 ( MGI:94903)
Sequence:sense strand is shown

>DLX3_probeA
ATGGCTGATCCTCAAGATGGGGGGGGGCTGACCTAGTCCCCACTGTTCTCCAGCCCTCATTAGGATTTGA
GGGTCCAAACCAAAGAAAACTCCCCCAAATGAGGGAACCTTTGATATCCAGGGCTTCCAAGGGAAGACTT
AAAGGAGCCATCCCTCCGCCCCTTCCCTGGGGAAAAGGATTGCCCATGTCCTTTTCCAAGGACCTGTTTC
TCCTTCCATGTACAGGACTTTGCACAAGTCTGGTTTTAAAAGCTGTTGAAACCTAGGACGACAATGGGCA
TTGTTAACAGTTAGGACCAAATCCACTCCTCTCTGGGCGGGCAGCTCTGCTCTGCCCTGACCCTGCTCAC
CCCTTCGTTTTGTTTTGTTTTGTTTTTCTCCCAGTAAGTTTGCAGTCTTGGGGGACGGGCTCAAACCCCA
ACTCTCTACAGAGATAGACCAGGCAAGACACCCCCCACTCAGACACCCCCTTCGTGGCAGCTTTGGAAAC
AGGTTGTCCGTTCTGC
nt 94940327 - nt 94940832 of NC_000077.4
Notes:These primers were used to generate the in vitro transcription template by PCR of mouse whole genomic DNA. They contain T7 or T3 promotor sequences and amplify a region corresponding to portions of the ORF and the 3'UTR. Forward 5' AATTAACCCTCACTAAAGGATGGCTGATCCTCAAGATGG (T3 sense) Reverse 5' TAATACGACTCACTATAGGGCAGAACGGACAACCTGTTT (T7 antisense)
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD-1
Age:11.5 dpc
Theiler Stage:TS19
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Jacqueline Ramsay, David R FitzPatrick
Principal investigator:David R FitzPatrick, Medical Research Council, Human Genetics Unit MRC Western General Hospital Crewe Road, Edinburgh, Scotland EH4 2XU
Submitted by:Jacqueline Ramsay, Medical Research Council, Human Genetics Unit MRC Western General Hospital Crewe Road, Edinburgh, Scotland EH4 2XU
Experiment type:screen
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceFACEBASE