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EMAGE:4329

Klf5 Kruppel-like factor 5 ( MGI:1338056)
TS18 (11 dpc)
in situ hybridisation

Data Images
EMAGE:4329 EMAGE:4329
Figure 4F. Copyright: This image is from Moore-Scott BA, Dev Dyn 2007 Jul;236(7):1997-2003 and is displayed with the permission of Wiley-Liss, Inc. who owns the Copyright. Figure 4F'. Copyright: This image is from Moore-Scott BA, Dev Dyn 2007 Jul;236(7):1997-2003 and is displayed with the permission of Wiley-Liss, Inc. who owns the Copyright.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: mg - midgut, hg - hindgut
Expression Pattern Description
Spatial Annotation:
EMAGE:4329Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4329_wholemount_strong_3D_1.wlz
4329_wholemount_possible_3D_1.wlz
4329_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4329_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
trunk somite
detected detected
otocyst
detected detected
hindgut epithelium
detected detected
nephric duct
detected detected
tail somite
detected detected
midgut
detected detected
regionalExpression is in the epithelium.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3715951
Entity Detected:Klf5, Kruppel-like factor 5 ( MGI:1338056)
Sequence:sense strand is shown

>MGI:3715951
GGATCTAGACATGCCCAGTTCGACAAACCAGACGGCAGTAATGGACACCCTTAATGTTTCTATGGCAGGC
CTTAACCCACACCCCTCTGCTGTTCCACAGACGTCAATGAAACAGTTCCAGGGCATGCCCCCTTGCACGT
ACACCATGCCAAGTCAGTTTCTTCCACAGCAGGCCACCTACTTTCCCCCGTCACCACCAAGCTCAGAGCC
TGGAAGTCCCGATAGACAAGCTGAGATGCTGCAGAATCTCACCCCACCTCCGTCCTATGCCGCTACAATT
GCTTCCAAACTGGCGATTCACAACCCAAATTTACCTGCCACTCTGCCAGTTAATTCGCCAACTCTCCCAC
CTGTCAGATACAACAGAAGGAGTAACCCGGATCTGGAGAAGCGACGTATCCACTTCTGCGATTATAATGG
TTGCACAAAAGTTTATACAAAGTCGTCTCACTTAAAAGCTCACCTGAGGACTCATACGGGCGAGAAGCCC
TACAAGTGCACCTGGGAGGGCTGCGACTGGAGGTTTGCCCGGTCGGATGAGCTGACCCGCCACTACAGGA
AGCACACGGGCGCCAAGCCGTTCCAGTGCATGGTGTGCCAACG
nt 988 - nt 1590 of NM_009769.4
Notes:The Klf5 probe template used in this study by Moore-Scott et al., 2007 [PMID:17576135] was generated by RT-PCR from isolated endoderm RNA using the following primers: F: 5'-gga tct aga cat gcc cag tt-3' (850F); R: 5'-cgt tgg cac acc atg cac t-3' (1451R).
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:ICR
Age:11 dpc
Theiler Stage:TS18
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:methanol
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Moore-Scott BA; Opoka R; Lin SC; Kordich JJ; Wells JM, 2007 [PMID:17576135] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1002/dvdy.21204] [ PMID:17576135] Moore-Scott BA, Opoka R, Lin SC, Kordich JJ, Wells JM 2007 Identification of molecular markers that are expressed in discrete anterior-posterior domains of the endoderm from the gastrula stage to mid-gestation. Dev Dyn (236):1997-2003
Links:MGI:3716119 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI