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EMAGE:4337

Dact1 dapper homolog 1, antagonist of beta-catenin (xenopus) ( MGI:1891740)
TS18 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4337
Figure 2D. Copyright: This image is from Fisher DA, Dev Dyn 2006 Jul 31;235(9):2620-2630 and is displayed with the permission of Wiley-Liss, Inc. who owns the Copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: D: tail bud (tb; vtm), limb buds (fl, hl); presomitic mesoderm (psm); spinal cord (sc); somites (so). Scale bar = 1.0 mm.
Expression Pattern Description
Spatial Annotation:
EMAGE:4337Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4337_wholemount_strong_3D_1.wlz
4337_wholemount_moderate_3D_1.wlz
4337_wholemount_weak_3D_1.wlz
4337_wholemount_possible_3D_1.wlz
4337_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4337_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
tail unsegmented mesenchyme
strong strong
tail somite
strong strong
tail mesenchyme
weak weak
regionalExpression is in the ventral mesoderm.
forelimb bud
detected detected
gradedExpression is in a proximal (low) to apical (high) gradient.
hindlimb bud
detected detected
gradedExpression is in a proximal (low) to apical (high) gradient.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3690218
Entity Detected:Dact1, dapper homolog 1, antagonist of beta-catenin (xenopus) ( MGI:1891740)
Sequence:sense strand is shown

>MGI:3690218
AAGAGCTCACAAGGTGTCAAGCCGGGCTGGGGGAATCCATGAAGGAAAGCAATCAGGCCTCCGCTGTTTC
TCCTAAAACAAGTCCTGGCAGAGGCCCTGTCGCCCCCGCAGAGAGCAAAGCCCTGCAGCTCCCGAAAAAG
ATGTCGCAGAAGAACAGCCTCCAGGCTGTGCCCGCGCTGGACAGGCCGGCCTTGGACTTCAAAAGCGAGG
GCTCATCTCAAAGCCTCGAGGAAGGGCATCTGGTGAAAGCTCAGTTCATTCCGGGGCAGCAGGCGGCCGC
CAGGCCTCACCGTGCACACAGGAACCCGGGTGTCGCAAGGAGCGCCACCTTGAAGGCCCGCGGCCAGGCA
GC
nt 1250 - nt 1601 of NM_021532.3
Notes:The Dact1 probe used in this study by Fisher et al, 2006 [PMID:16881060] corresponded to nucleotides 1250-1601 (relative to the translational start). The same pattern was also seen using a second probe that spanned nt 316-692 (relative to the translational start). Editors Note. The translational start in the Dact1 cDNA RefSeq NM_021532.3 is at position 1.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD-1
Age:10.5 dpc
Theiler Stage:TS18
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Fisher DA; Kivimae S; Hoshino J; Suriben R; Martin PM; Baxter N; Cheyette BN, 2006 [PMID:16881060] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1002/dvdy.20968] [ PMID:17013874] Suriben R, Fisher DA, Cheyette BN 2006 Dact1 presomitic mesoderm expression oscillates in phase with Axin2 in the somitogenesis clock of mice. Dev Dyn (235):3177-83
 [ doi:10.1002/dvdy.20917] [ PMID:16881060] Fisher DA, Kivimae S, Hoshino J, Suriben R, Martin PM, Baxter N, Cheyette BN 2006 Three Dact gene family members are expressed during embryonic development and in the adult brains of mice. Dev Dyn (235):2620-30
Links:MGI:3691489 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI