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EMAGE:4338

Armcx2 armadillo repeat containing, X-linked 2 ( MGI:1914666)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4338
Figure 2I. Copyright: This image is from Smith CA, Dev Dyn 2005 May;233(1):188-93 and is displayed with the permission of Wiley-Liss, Inc. who owns the Copyright.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: arrow, somites.
Expression Pattern Description
Spatial Annotation:
EMAGE:4338Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4338_wholemount_strong_3D_1.wlz
4338_wholemount_moderate_3D_1.wlz
4338_wholemount_possible_3D_1.wlz
4338_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4338_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
trunk somite
detected detected
forebrain
detected detected
midbrain
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3577412
Entity Detected:Armcx2, armadillo repeat containing, X-linked 2 ( MGI:1914666)
Sequence:sense strand is shown

>MGI:3577412
GCCGTGTGGAACTGTCTTTATCCGTCTACCTTGAGGCTCTGCAACTGCGAGCACAGTGGAAATTGTTCCT
GATCCCAGGAAGCTGGTCCAGCTCAGCTTGCAGCCTCCGAAGAACTTTCAGACCCGAGCTTGAGATCCTC
GATTGGACTGGTGTATACATTTCGCTTAAGCAAGCCAGTTCCACAACCTGGATCAGAGACTCACTCCAGG
TGTACCATGAGCCGAGCTCGGGATGCTGGCTGTGTAGCTGCTGGAATAGTGATTGGGGCGAGTGCCTGGT
ACTGTGTCTACAAATATACTAGGGGAAAAGACCAGAAGAAGAAGAGGCTGACCAAGCCCAAGAACCGGGC
CTCTGTGGGTACTGGAAGCAGGGCTAGAGCGGGCCTCAGAGCTGGATTCACAATTGACCTTGGGCCAGGA
TTCAGTCCCCCAAACCCCGTCGATATTGAGATAATGAACAAGGCCCAAGGAGAAGCCTCCAATCTTGCCA
CCACCGTAGCTGAAGAAGTGGCACCAGCTGCACCCAGTCCTAAGGTTCAGAATGGGGCAGAAAGTAAGGT
CCAGGAGTTAAATGGGGCTAAAACTGAGGCTAATCTGGAATCAGTAGTCATGCCTTCAGCTACCTGTACA
GTTACACCTCCTCCCAAGGTGGCAGGGGGTCTCACTGCTGCAGAGGCTCCAGAAATCATAGGAGCTCCCA
AAGTGCTGGAAGCTCCCAGCACCACAGAGGCTTCTGGGGCAGTAGCAGCCCCTGGACCAACAGTATCTCC
AATGATAGCCCAGACTCCTGGGCCAGTGGTACCTTCGCCA
nt 595 - nt 1404 of NM_026139.3
Notes:The Armcx2 (Alex2) probe used in this study by Smith et al., 2005 [PMID:15759267] is described as follows: "800-bp sequence encompassing much of the mouse Alex2 unique region and 5' untranslated region (UTR, Fig. 1) was used as a probe. PCR primers used to generate this probe were as follows: AlexA, 5'-GCC GTG TGG AAC TGT CTT TAT CCG-3'; and AlexB, 5'-TGG CGA AGG TAC CAC TGG CC-3'".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Smith CA; McClive PJ; Sinclair AH, 2005 [PMID:15759267] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1002/dvdy.20309] [ PMID:15759267] Smith CA, McClive PJ, Sinclair AH 2005 Temporal and spatial expression profile of the novel armadillo-related gene, Alex2, during testicular differentiation in the mouse embryo. Dev Dyn (233):188-93
Links:MGI:3577624 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI