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EMAGE:4342

Angptl1 angiopoietin-like 1 ( MGI:1919963)
TS16 (9.5 dpc)
in situ reporter

Data Images
EMAGE:4342
Figure 3A. Copyright: This image is from Lai DM, Dev Dyn 2007 Sep;236(9):2643-52 and is displayed with the permission of Wiley-Liss, Inc. who owns the Copyright.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Editor's note: This specimen is homozygous null for Angptl1, however the authors report that such mice are "morphologically indistinguishable from their heterozygous and wildtype littermates" and have a "normal phenotype and reproductive capacity".
Expression Pattern Description
Spatial Annotation:
EMAGE:4342Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4342_wholemount_strong_3D_1.wlz
4342_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4342_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
regionalExpression is in the head, in the craniocervical junction.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ reporter
Entity Detected:Angptl1, angiopoietin-like 1 ( MGI:1919963)
Notes:The Angptl1 gene targeting (and expression tracking) strategy used in this study by Lai et al., 2007 [PMID:17676644] was as follows: "Angptl1 genomic DNA was isolated from a 129/SvJ mouse genomic library and subcloned into the pBluescript SK vector (Stratagene). A 1-kb upstream fragment and a 9.5-kb downstream fragment of the angptl1 gene were subcloned into XhoI and NotI sites of the pNZGKII vector, respectively. The coding region, including the ATG start site (at exon 2) of the angptl1 gene, was replaced by an nLacZ/neo cassette. The targeting vector was linearized with NotI and electroporated into ES cells".
Labelled with:LacZ
Specimen
Organism:mouse
Strain:involves: C57BL/6J
Age:9.5 dpc
Theiler Stage:TS16
Mutations:Angptl1tm1Hung/Angptl1tm1Hung
Preparation:wholemount
Procedures
Fixation:paraformaldehyde
General Information
Authors:Lai DM; Tu YK; Hsieh YH; Hsu WM; Lee CC; Cheng WC; Hsieh FJ; Li H, 2007 [PMID:17676644] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1002/dvdy.21262] [ PMID:17676644] Lai DM, Tu YK, Hsieh YH, Hsu WM, Lee CC, Cheng WC, Hsieh FJ, Li H 2007 Angiopoietin-like protein 1 expression is related to intermuscular connective tissue and cartilage development. Dev Dyn (236):2643-52
Links:MGI:3722837 same experiment
 MGI:3721928 allele description
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE