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EMAGE:4343

Dact2 dapper homolog 2, antagonist of beta-catenin (xenopus) ( MGI:1920347)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4343
Figure 2E. Copyright: This image is from Fisher DA, Dev Dyn 2006 Jul 31;235(9):2620-2630 and is displayed with the permission of Wiley-Liss, Inc. who owns the Copyright.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: somites (so), otic vesicle epithelium (ov). Scale bar = 1.0 mm. Inset: Another example of caudal somite expression plus no expression detected in the forebrain (fb) or mandibular arch (md; obscured in E)
Expression Pattern Description
Spatial Annotation:
EMAGE:4343Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4343_wholemount_strong_3D_1.wlz
4343_wholemount_moderate_3D_1.wlz
4343_wholemount_possible_3D_1.wlz
4343_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4343_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
tail somite
detected detected
regionalExpression is in the caudal-most somites and in the most recently-formed somite, a weaker signal is in the next two youngest somites.
otocyst
detected detected
forebrain
not detected not detected
homogeneous
1st branchial arch mandibular component
not detected not detected
homogeneous
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3691490
Entity Detected:Dact2, dapper homolog 2, antagonist of beta-catenin (xenopus) ( MGI:1920347)
Sequence:sense strand is shown

>MGI:3691490
TCCTTGTCCCCGTAGTGGTGGCCCAGAGGGAAAGTTACCCAACGTCACCCCAAGCGTTCTTCCCAATGGA
GGCAGCTCTCCTCAGCTCAGCAGCCAGGCGGAAGCAGCGCAGGTGGCAGTCCACCATGGAGATCTCAGCC
AAAGCCGGTTCAGTCAGCCAACCTGGGCCCAGCATGGGGCTCCCTAGGTCCCCAGCCAAGAGAGGAAGTG
GTCCCAGGGCCCAGAGTAGGCCCACACTTGCCCGTCAGGATGCCTGTGCGAGGTGTGAGTCGGACCCCCC
GGAGCACTCTGCAGACTGCGCCTCACTCTACCACTCCACCATTGC
nt 2005 - nt 2329 of AY297430.1
Notes:The Dact2 probe used in this study by Fisher et al., 2006 [PMID:16881060] spanned "nt 1639-1963 (nucleotide numbers relative to translational start)". A second probe spanning nt 610-1080 (relative to the translational start), also gave a similar pattern. Editors Note: The Dact2 (Dpr2) cDNA sequence AY297430.1 (2723bp) was submitted to GenBank in conjunction with this publication, the translational start site of which is at position 366.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Fisher DA; Kivimae S; Hoshino J; Suriben R; Martin PM; Baxter N; Cheyette BN, 2006 [PMID:16881060] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1002/dvdy.20917] [ PMID:16881060] Fisher DA, Kivimae S, Hoshino J, Suriben R, Martin PM, Baxter N, Cheyette BN 2006 Three Dact gene family members are expressed during embryonic development and in the adult brains of mice. Dev Dyn (235):2620-30
Links:MGI:3691491 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI