Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:4354

Dact3 dapper homolog 3, antagonist of beta-catenin (xenopus) ( MGI:3654828)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4354
Figure 2F. Copyright: This image is from Fisher DA, Dev Dyn 2006 Jul 31;235(9):2620-2630 and is displayed with the permission of Wiley-Liss, Inc. who owns the Copyright.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: facial mesenchyme (fm), branchial arch mesenchyme: (mx) maxillary, (md) mandibular, (hy) hyoid, limb buds (fl, hl), otic vesicle epithelium (ov), spinal cord (sc), tail bud (tb). Scale bar = 1.0 mm.
Expression Pattern Description
Spatial Annotation:
EMAGE:4354Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4354_wholemount_strong_3D_1.wlz
4354_wholemount_moderate_3D_1.wlz
4354_wholemount_weak_3D_1.wlz
4354_wholemount_possible_3D_1.wlz
4354_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4354_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
branchial arch
strong strong
regionalExpression in mesenchyme.
limb
strong strong
regionalExpression in mesenchyme.
trunk somite
strong strong
regionalExpression is in maturing somites.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3691492
Entity Detected:Dact3, dapper homolog 3, antagonist of beta-catenin (xenopus) ( MGI:3654828)
Sequence:sense strand is shown

>MGI:3691492
GGAGCAGCTGGAGGCCCTGCCTGGGCTCATCTGGGACCTGGGCCAGCAGCTGGGAGACCTGAGTCTGGAG
TCTGGTGGCCTGGACCAGGAGAGTGGGCGCAGCTCAGGCTTCTATGAAGACCCCAGTTCTACTGGAGGCC
CAGATTCACCACCCTCCACCTTCTGCGGGGACAGTGGCTTCTCCGGATCTGGCTCCTATGGACGCCTGGG
TCCCTCGGACCCCCGGGGCATCTATGCCAGTGAGAGACCCAAGTCCTTAGGCGACGCCAGTCCCAGCGCC
CCAGAGTCGGTGGGTGCCCGGGTTGCAGTGCCTCGATCCTTCTCAGCTCCCTATCCGACGGCAGCAGCCG
GAGCTGAGACCTGCTCATC
nt 348 - nt 716 of DQ832319.1
Notes:The Dact3 probe used in this study by Fisher et al., 2006 [PMID:16881060] covered nt 239-607 relative to the translational start site. Editors note: The authors also used three other Dact3 probes nt 813-1153, nt 1162-1641, nt 1643-1910 (relative to the translational start site), all of which gave similar patterns. The Dact3 cDNA sequence DQ832319 was submitted to GenBank by the authors, the translational start site of which is at nt 109.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Fisher DA; Kivimae S; Hoshino J; Suriben R; Martin PM; Baxter N; Cheyette BN, 2006 [PMID:16881060] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1002/dvdy.20917] [ PMID:16881060] Fisher DA, Kivimae S, Hoshino J, Suriben R, Martin PM, Baxter N, Cheyette BN 2006 Three Dact gene family members are expressed during embryonic development and in the adult brains of mice. Dev Dyn (235):2620-30
Links:MGI:3691493 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI