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EMAGE:4369

Ipmk inositol polyphosphate multikinase ( MGI:1916968)
TS16 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:4369 EMAGE:4369
Figure 3G. Copyright: This image is from Frederick JP, Proc Natl Acad Sci U S A 2005 Jun 14;102(24):8454-9 [PMID:15939867] . Copyright 2005 National Academy of Sciences, U.S.A. Figure 3H. Copyright: This image is from Frederick JP, Proc Natl Acad Sci U S A 2005 Jun 14;102(24):8454-9 [PMID:15939867] . Copyright 2005 National Academy of Sciences, U.S.A.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: ba -branchial arch, nt - neural tube, lb - limb bud. *** NOTE: G has been hybridised with antisense probe whereas H is a negative control, hybridised with sense probe.***
Expression Pattern Description
Spatial Annotation:
EMAGE:4369Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4369_wholemount_strong_3D_1.wlz
4369_wholemount_moderate_3D_1.wlz
4369_wholemount_weak_3D_1.wlz
4369_wholemount_possible_3D_1.wlz
4369_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4369_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
branchial arch
strong strong
neural tube
strong strong
hindlimb bud
strong strong
regional
trunk somite
strong strong
embryo
strong strong
regionalExpression in head.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3614406
Entity Detected:Ipmk, inositol polyphosphate multikinase ( MGI:1916968)
Notes:The Ipmk (Ipk2) probes used in this study by Frederick et al., 2005 [PMID:15939867] were generated thus: "antisense and sense digoxigenin (DIG)-labeled riboprobes were generated by T7 RNA polymerase from two oppositely oriented clones of the Ipk2 ORF in pCR2.1 TOPO".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS16
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Frederick JP; Mattiske D; Wofford JA; Megosh LC; Drake LY; Chiou ST; Hogan BL; York JD, 2005 [PMID:15939867] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1073/pnas.0503706102] [ PMID:15939867] Frederick JP, Mattiske D, Wofford JA, Megosh LC, Drake LY, Chiou ST, Hogan BL, York JD 2005 An essential role for an inositol polyphosphate multikinase, Ipk2, in mouse embryogenesis and second messenger production. Proc Natl Acad Sci U S A (102):8454-9
Links:MGI:3614407 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI