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EMAGE:4381

Bcor BCL6 interacting corepressor ( MGI:1918708)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:4381 EMAGE:4381
Fig3C. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.modgep.2007.01.006] Gene Expr Patterns 7: 550-7, Wamstad JA; Bardwell VJ, Characterization of Bcor expression in mouse development. Copyright 2007

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: BA - branchial arches; LB - limb bud. Note that C has been treated with anti-sense probe whereas F is a sense control.
Expression Pattern Description
Spatial Annotation:
EMAGE:4381Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4381_wholemount_strong_3D_1.wlz
4381_wholemount_moderate_3D_1.wlz
4381_wholemount_weak_3D_1.wlz
4381_wholemount_possible_3D_1.wlz
4381_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4381_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
branchial arch
strong strong
limb
strong strong
embryo
detected detected
There is less robust expression throughout the rest of the embryo.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3714011
Entity Detected:Bcor, BCL6 interacting corepressor ( MGI:1918708)
Sequence:sense strand is shown

>MGI:3714011
ATCGATGGCCTGGCAGCACTGAGCATGGACCGCACAGGCCTCATCCGGGAAGGGCTCCGGGTCCCTGGAA
ACATCGTTTATTCTGGCTTGTGTGGATTGGGCTCAGAAAAAGGTCGGGAGGCTACCCCAAGCTCTCTCAG
TGGCCTTGGGTTTTCTTCGGAAAGAAATCCAGAGATGCAATTCAAACCGAATACTCCCGAGACAGTGGAG
GCTTCTGCTGTCTCTGGAAAACCCCCAAATGGCTTCAGTGCTATATACAAAACACCGCCTGGGATACAAA
AGAGCGCTGTAGTTACAGCGGAAAGCTTGAGTTTGGATAGGCCTGCCAGTGACAAACAGAGCCCTCTCAA
CATCAATGGTGCTAGTTACCTGCGGCTGCCCTGGGTCAACCCTTACATGGAGGGGGCCACTCCAGCCATA
TACCCTTTCCTCGACTCGCCAAATAAGTATTCACTGAATATGTACAAGGCCTTGCTACCTCAGCAGTCCT
ACGGCTTGGCCCAGCCACTGTATTCTCCTGTCTGCACCAGTGGGGAACGCTTCCTCTACCTGCCACCCCC
ACACTATGTCAACCCTCACATCCCCTCCTCCTTGGCTTCACCCATGAGGCTCTCCACACCTTCAGCCTCT
GCGGCCATTCCACCTCTCGTCCACTGCTCAGACAAAAGCCTCCCATGGAAGATGGGCGTCAACCCTGGGA
ACCCAGTAGATTCCCACAGTTACCCCCACATCCAAAACAGTAAACAGCCCCGGGTGACCTCTGCCAAGGC
AGTCAATAGTGGCCTGCCAGGGGACACAGCTCTCCTGTTGCCTCCTTCACCTCGGCCCTCAGCCCGGGTC
CACCTTCCAACTCAGCCCGCTGCAGAGACATACTCCGAGTTCCACAAGCACTATCCCAGGATCTCCACCT
CTCCCTCAGTCACCCTGACGAAGCCATACATGACAGCCAATAGCGAGTTCTCTACATCCAGGCTGTCCAA
T
nt 280 - nt 1260 of NM_029510.2
Notes:The Bcor probe template used in this study by Wamstad & Bardwell, 2007 [PMID:17344103] is described as follows: An amplicon containing nucleotides 280-1294 of the Bcor open reading frame was generated by PCR from a bacterial artificial chromosome containing the mouse Bcor gene using the following primers, forward: 5'-TACGGGTACCATCGATGGCCTGGCAGCACT-3' and reverse: 5'-CATGTCTAGATTGGACAGCCTGGATGTAGAG-3'. The PCR amplicon was gel purified and directly cloned into the Kpn and Xba restriction sites of a pBluescript KS+ vector. Editors note: although the authors state that the probe contains nucleotides 280-1294 of the Bcor ORF, when these primer sequences are BLASTed against the 3 Bcor mRNA RefSeq sequences: NM_029510.2 (transcript variant a); NM_175044.2 (transcript variant b) and NM_175045.2 (transcript variant c), the resulting start and end points for the PCR amplified fragment are nt280-1260, nt280-1260 and nt466-1446 respectively, the intervening sequence of which is identical for all three.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD-1
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Wamstad JA; Bardwell VJ, 2007 [PMID:17344103] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/j.modgep.2007.01.006] [ PMID:17344103] Wamstad JA, Bardwell VJ 2007 Characterization of Bcor expression in mouse development. Gene Expr Patterns (7):550-7
Links:MGI:3714289 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI