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EMAGE:4383

Sbf2 SET binding factor 2 ( MGI:1921831)
TS16 (10.0 dpc)
in situ hybridisation

Data Images
EMAGE:4383 EMAGE:4383
Fig3B (anti[-sense]). Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.modgep.2006.04.005] Gene Expr Patterns 6: 978-84, Kirfel J; Senderek J; Moser M; Roper A; Stendel C; Bergmann C; Zerres K; Buettner R, Cloning, expression and characterization of the murine orthologue of SBF2, the gene mutated in Charcot-Marie-Tooth disease type 4B2. Copyright 2006 Fig3B (sense). Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.modgep.2006.04.005] Gene Expr Patterns 6: 978-84, Kirfel J; Senderek J; Moser M; Roper A; Stendel C; Bergmann C; Zerres K; Buettner R, Cloning, expression and characterization of the murine orthologue of SBF2, the gene mutated in Charcot-Marie-Tooth disease type 4B2. Copyright 2006

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Images of embryos treated with both anti-sense and sense (i.e. control) probes are shown.
Expression Pattern Description
Spatial Annotation:
EMAGE:4383Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4383_wholemount_strong_3D_1.wlz
4383_wholemount_moderate_3D_1.wlz
4383_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4383_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
embryo
strong strong
regionalExpression is in the craniofacial region.
branchial arch
strong strong
limb
strong strong
future brain
detected detected
neural tube
detected detected
heart
not detected not detected
homogeneous
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3664261
Entity Detected:Sbf2, SET binding factor 2 ( MGI:1921831)
Notes:The Sbf2 probe template used in this study by Kirfel et al., 2006 [PMID:16750429] is described as either "a 900 or 1291 bp fragment obtained from the murine cDNA (nt 1200-2100 or nt 2970-4261) which were amplified by PCR and subcloned into pCRIITOPO (Invitrogen)." Editors note: it is unspecified which of these probe templates was used in this example.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.0 dpc
Theiler Stage:TS16
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Kirfel J; Senderek J; Moser M; Roper A; Stendel C; Bergmann C; Zerres K; Buettner R, 2006 [PMID:16750429] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/j.modgep.2006.04.005] [ PMID:16750429] Kirfel J, Senderek J, Moser M, Roper A, Stendel C, Bergmann C, Zerres K, Buettner R 2006 Cloning, expression and characterization of the murine orthologue of SBF2, the gene mutated in Charcot-Marie-Tooth disease type 4B2. Gene Expr Patterns (6):978-84
Links:MGI:3664285 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI