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EMAGE:4388

Kat5 K(lysine) acetyltransferase 5 ( MGI:1932051)
TS16 (10.0 dpc)
in situ hybridisation

Data Images
EMAGE:4388 EMAGE:4388
Fig2D. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.modgep.2007.03.005] Gene Expr Patterns 7: 657-65, Thomas T; Loveland KL; Voss AK, The genes coding for the MYST family histone acetyltransferases, Tip60 and Mof, are expressed at high levels during sperm development. Copyright 2007 Fig2F. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.modgep.2007.03.005] Gene Expr Patterns 7: 657-65, Thomas T; Loveland KL; Voss AK, The genes coding for the MYST family histone acetyltransferases, Tip60 and Mof, are expressed at high levels during sperm development. Copyright 2007

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: vNT - Ventral neural tube, vFb - ventral forebrain. D is hybridised with anti-sense probe, F with sense probe.
Expression Pattern Description
Spatial Annotation:
EMAGE:4388Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4388_wholemount_strong_3D_1.wlz
4388_wholemount_moderate_3D_1.wlz
4388_wholemount_possible_3D_1.wlz
4388_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4388_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
neural tube
strong strong
regionalExpression is in the ventral neural tube.
future forebrain
strong strong
regionalExpression is in the ventral forebrain.
future hindbrain
strong strong
regionalExpression is in the ventral hindbrain.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3713367
Entity Detected:Kat5, K(lysine) acetyltransferase 5 ( MGI:1932051)
Sequence:sense strand is shown

>MGI:3713367
CCCCAGCCTCGGTTTTCCCTCAGAATGGGTCAGCCCGTAGGGCAGTGGCAGCCCAGCCTGGACGGAAGCG
GAAATCTAATTGCTTGGGCACTGATGAGGATTCTCAGGACAGCTCAGATGGAATACCGTCAGCACCACGA
ATGACTGGCAGTCTGGTGTCTGACCGGAGCCACGACGACATTGTCACCCGGATGAAGAACATTGAGTGTA
TTGAGCTTGGCCGGCACCGCCTCAAGCCGTGGTACTTCTCCCCGTACCCACAAGAGCTTACCACGCTACC
CGTCCTCTACCTGTGCGAATTTTGCCTCAAATATGGCCGTAGCCTCAAGTGTCTGCAACGCCACTTGACC
AAATGTGATCTTCGGCACCCTCCAGGCAATGAAATTTACCGCAAGGGCACCATCTCCTTTTTTGAGATTG
ATGGACGGAAAAACAAGAGTTACTCACAAAACCTGTGTCTTCTGGCCAAGTGTTTCCTGGACCACAAAAC
ACTGTACTATGACACTGACCCCTTCCTCTTCTACGTAATGACGGAGTATGACTGCAAAGGTTTCCACATC
GTGGGCTACTTCTCCAAGGAAAAGGAATCCACAGAAGATTACAATGTGGCCTGCATCTTGACTCTGCCTC
CCTACCAGCGCCGGGGCTATGGCAAGCTGCTTATTGAGTTCAGCTATGAACTCTCGAAAGTAGAAGGGAA
GACCGGAACTCCTGAGAAACCCCTGTCAGATCTTGGCCTCCTATCCTACCGAAGTTACTGGTCCCAAACC
ATCTTGGAGATCCTGATGGGGCTGAAGTCGGAGAGCGGGGAGAGG
nt 494 - nt 1308 of NM_178637.1
Notes:The Htatip (Tip60) probe template used in this study by Thomas et al., 2007 [PMID:17517537] is described as follows: "Tip60 and Mof cDNA probes were generated by RT-PCR and verified by sequencing. The Tip60 probe consisted of bases 494-1308 (GenBank Accession No.: NM_178637) cloned into the EcoRV site of Bluescript KS+ (Stratagene)."
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD-1
Age:10.0 dpc
Theiler Stage:TS16
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Thomas T; Loveland KL; Voss AK, 2007 [PMID:17517537] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/j.modgep.2007.03.005] [ PMID:17517537] Thomas T, Loveland KL, Voss AK 2007 The genes coding for the MYST family histone acetyltransferases, Tip60 and Mof, are expressed at high levels during sperm development. Gene Expr Patterns (7):657-65
Links:MGI:3713392 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI