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EMAGE:4398

Pcdh19 protocadherin 19 ( MGI:2685563)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4398
Fig1B. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.modgep.2006.03.001] Gene Expr Patterns 6: 893-9, Gaitan Y; Bouchard M, Expression of the delta-protocadherin gene Pcdh19 in the developing mouse embryo. Copyright 2006

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: ba - branchial arch; fb - forebrain; lb - limb bud; mb - midbrain; nd - nephric duct; ov - otic vesicle; psm - presomitic mesoderm.
Expression Pattern Description
Spatial Annotation:
EMAGE:4398Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4398_wholemount_strong_3D_1.wlz
4398_wholemount_moderate_3D_1.wlz
4398_wholemount_weak_3D_1.wlz
4398_wholemount_possible_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4398_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
forebrain
detected detected
hindbrain
detected detected
regionalExpression is in a discreet domain.
midbrain
detected detected
1st branchial arch
detected detected
regionalexpression in mesenchyme
2nd branchial arch mesenchyme
detected detected
forelimb bud mesenchyme
detected detected
hindlimb bud mesenchyme
detected detected
otocyst
detected detected
tail unsegmented mesenchyme
detected detected
nephric duct
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3663670
Entity Detected:Pcdh19, protocadherin 19 ( MGI:2685563)
Sequence:sense strand is shown

>MGI:3663670
CACCAAGCAGAAGATTGACCGAGACCTGCTGTGCCGCCAGAGCCCCAAGTGTATCATCTCGCTCGAGGTC
ATGTCCAGCTCAATGGAGATCTGTGTGATTAAGGTGGAGATCAAAGACCTGAACGACAATGCTCCCAGTT
TTCCGGCCGCACAGATAGAGCTGGAGATCTCTGAGGCAGCCAGCCCCGGCACCCGCATCCCACTGGACAG
TGCATATGACCCGGACTCCGGCAGCTTTGGTGTGCAGACCTACGAGCTCACGCCCAACGAGCTGTTTGGC
CTGGAAATAAAGACGCGGGGTGACGGTTCGCGCTTTGCTGAACTGGTGGTGGAGAAGAGCCTGGACCGTG
AGACACAGTCACATTACAGCTTTCGCATTACGGCTCTCGACGGAGGCGACCCACCACACATGGGCACAGT
TGGCCTCAGCATCAAGGTGACCGATTCGAATGACAACAACCCAGTGTTTGGCGAGTCCACCTACTCAGTG
AGCGTGCCTGAAAATTCACCTCCCAATACACCCGTCATCCGCCTCAATGCCAGCGATCCAGATGAGGGCA
CCAATGGCCAAGTGGTCTATTCCTTCTATGGTTATGTCAATGACCGTACGCGTGAACTCTTCCAAATTGA
CCCACACAGCGGCCTGGTCACCGTCACCGGTGCGCTAGACTATGAAGAGGGCCATGTGTACGAACTGGAT
GTGCAAGCCAAAGACCTGGGGCCCAACTCTATCCCTGCACACTGCAAAGTCACAGTCAGCGTACTTGATA
CTAATGACAACCCGCCAATTATCAACCTCCTGTCGGTCAATAGCGAGCTTGTGGAGGTGAGCGAGAGCGC
CCCCCCGGGCTATGTGATTGCCCTGGTTCGGGTGTCTGATCGCGACTCCGGGCTCAATGGACGTGTGCAG
TGCCGCTTGCTGGGCAATGTCCCCTTTCGACTGCAGGAGTATGAGAGCTTCTCCACTATTCTTGTGGATG
GGAGGCT
nt 1963 - nt 2949 of XM_205287.4
Notes:The Pcdh19 probe used in this study by Gaitan & Bouchard, 2006 [PMID:16682261] is described as follows: "Pcdh19 cRNA probe was generated by RT-PCR from E17.5 kidney cDNA using the following primers: 5'-CACCAAGCAGAAGATTGACCGAG-3' and 5'-GCCTCCCATCCACAAGAATAGTG-3'. The 986 bp PCR product, corresponding to positions 1963-2949 of the reference sequence XM_205287 (exon 1), was cloned in pGEM-T-easy and used for digoxigenin-dUTP cRNA probe synthesis, following linearization." Editors note: The co-ordinates given by the authors for XM_205287 refer to version 4 (XM_205287.4).
Chemistry:RNA
Strand:sense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Gaitan Y; Bouchard M, 2006 [PMID:16682261] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/j.modgep.2006.03.001] [ PMID:16682261] Gaitan Y, Bouchard M 2006 Expression of the delta-protocadherin gene Pcdh19 in the developing mouse embryo. Gene Expr Patterns (6):893-9
Links:MGI:3663740 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI