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EMAGE:4403

Csf1r colony stimulating factor 1 receptor ( MGI:1339758)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4403
Fig1A. Lichanska AM; Browne CM; Henkel GW; Murphy KM; Ostrowski MC; McKercher SR; Maki RA; Hume DA, "Differentiation of the mononuclear phagocyte system during mouse embryogenesis: the role of transcription factor PU.1." Blood 1999 Jul 1;94(1):127-38 [PMID:10381505] .

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4403Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4403_wholemount_strong_3D_1.wlz
4403_wholemount_moderate_3D_1.wlz
4403_wholemount_weak_3D_1.wlz
4403_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4403_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
spottedExpression is described as a very extensive speckled pattern throughout the embryo. Each spot is a single-labelled cell. Labeled cells are particularly concentrated in the head, around the branchial arches, and along the dorsal midline, although this is not evident in a single focal plane.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Csf1r cloneA
Entity Detected:Csf1r, colony stimulating factor 1 receptor ( MGI:1339758)
Notes:The Csf1r (c-fms) probe template used in this study by Lichanska et al., 1999 [PMID:10381505] is described as an ~800bp fragment cloned in pGEM2, and cut with either NdeI or HindIII for generation of an antisense probe.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD-1 or Swiss Quackenbush
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Lichanska AM; Browne CM; Henkel GW; Murphy KM; Ostrowski MC; McKercher SR; Maki RA; Hume DA, 1999 [PMID:10381505] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:10381505] Lichanska AM, Browne CM, Henkel GW, Murphy KM, Ostrowski MC, McKercher SR, Maki RA, Hume DA 1999 Differentiation of the mononuclear phagocyte system during mouse embryogenesis: the role of transcription factor PU.1. Blood (94):127-38
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE