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EMAGE:4405

Lyz2 lysozyme 2 ( MGI:96897)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4405
Fig5A. Lichanska AM; Browne CM; Henkel GW; Murphy KM; Ostrowski MC; McKercher SR; Maki RA; Hume DA, "Differentiation of the mononuclear phagocyte system during mouse embryogenesis: the role of transcription factor PU.1." Blood 1999 Jul 1;94(1):127-38 [PMID:10381505] .

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
The authors state that the band of 'expression' observed in the heart in this assay is due to nonspecific (reagent) trapping.
Expression Pattern Description
Spatial Annotation:
EMAGE:4405Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4405_wholemount_strong_3D_1.wlz
4405_wholemount_moderate_3D_1.wlz
4405_wholemount_possible_3D_1.wlz
4405_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4405_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
liver
detected detected
spottedExpression was restricted to sparse large cells in the liver.
forelimb bud
detected detected
spottedA small number of stained cells can be seen in this focal plane in the forelimb bud.
embryo
detected detected
spottedFew stained cells are detected elsewhere in the embryo apart from the liver and limb bud.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Lyz2 cloneA
Entity Detected:Lyz2, lysozyme 2 ( MGI:96897)
Notes:The Lyz2 (lysozyme) probe template used in this study by Lichanska et al., 1999 [PMID:10381505] is described as a 300bp fragment cloned in pBS, which was cut with PstI prior to generation of antisense probe. The clone was obtained from R. Renkawitz (Max Planck Institute, Martinsried, Germany).
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD-1 or Swiss Quackenbush
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Lichanska AM; Browne CM; Henkel GW; Murphy KM; Ostrowski MC; McKercher SR; Maki RA; Hume DA, 1999 [PMID:10381505] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:10381505] Lichanska AM, Browne CM, Henkel GW, Murphy KM, Ostrowski MC, McKercher SR, Maki RA, Hume DA 1999 Differentiation of the mononuclear phagocyte system during mouse embryogenesis: the role of transcription factor PU.1. Blood (94):127-38
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE