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EMAGE:4406

Msr1 macrophage scavenger receptor 1 ( MGI:98257)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4406
Fig3C. Lichanska AM; Browne CM; Henkel GW; Murphy KM; Ostrowski MC; McKercher SR; Maki RA; Hume DA, "Differentiation of the mononuclear phagocyte system during mouse embryogenesis: the role of transcription factor PU.1." Blood 1999 Jul 1;94(1):127-38 [PMID:10381505] .

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4406Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4406_wholemount_strong_3D_1.wlz
4406_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4406_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
heart
detected detected
liver
detected detected
The first labeled cells can be detected in the liver at this time
limb
detected detected
not shown
embryo
detected detected
spottedA few positively staining cells were detected all over the body, but most at this time are detected in the liver.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Msr1 cloneA
Entity Detected:Msr1, macrophage scavenger receptor 1 ( MGI:98257)
Notes:The Msr1 (MSR/scavenger receptor) probe template clone used in this study by Lichanska et al., 1999 [PMID:10381505] is described as a 1,444bp fragment cloned into pJA18 which was cut with HindIII prior to generation of the antisense probe. This clone was obtained from M. Krieger (Massachusetts Institute of Technology, Boston, MA)
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD-1 or Swiss Quackenbush
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Lichanska AM; Browne CM; Henkel GW; Murphy KM; Ostrowski MC; McKercher SR; Maki RA; Hume DA, 1999 [PMID:10381505] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:10381505] Lichanska AM, Browne CM, Henkel GW, Murphy KM, Ostrowski MC, McKercher SR, Maki RA, Hume DA 1999 Differentiation of the mononuclear phagocyte system during mouse embryogenesis: the role of transcription factor PU.1. Blood (94):127-38
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE