EMAGE mouse gene expression spatial database

EMAGE:4408

Itga4 integrin alpha 4 ( MGI:96603)
TS16 (9.5 dpc)
in situ hybridisation

Data Images


Fig1k. Copyright: This image is from [doi:10.1002/dvdy.20136] Bajanca F; Luz M; Duxson MJ; Thorsteinsdottir S, "Integrins in the mouse myotome: developmental changes and differences between the epaxial and hypaxial lineage." Dev Dyn 2004 Oct;231(2):402-15, and is displayed with the permission of Wiley-Liss, Inc. who owns the Copyright.

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

Image annotations: Yellow arrow = the first clearly epithelial somite, black arrow = somite IV.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

Download individual expression domains:

Download all expression domains:

Find spatially similar wholemount expression patterns:

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

trunk somite

detected

regional

Itga4 expression is detected more caudally than Mlc1a at this stage (i.e. at somite IV).

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	Itga4 cloneA
Entity Detected:	Itga4, integrin alpha 4 ( MGI:96603)
Sequence:	sense strand is shown >Itga4 cloneA CAACAGCAAAAGCAATGATGACTGAAGACTTCTACACTGAGAGAACTGAAAAACTCAGGTTAGGAAAAAG AAATCCTGTTCAGAAGACCCGTCAGAATTTCTTCTTTTTTTCCATGTGCTTATGATTTTGTGACATACTC TTAGTGCAGGGGAAATCTTCAAGAAAGAAGCTACCCAAAGGTGGCTTGTCAGCTTCGGTGGATGAGTGAA GCAAAACACTGAAGCTCTGGATGTACCGGAGAGGTGACCTGTTTAAGACAACTTAAAGCTAGAGAGAATC CAGACTCAGCAGGGCCGACTTAAAGGGAATGATTTTTCAACATCACTGATGAAGTGGCCATCTCAGTGAA ATGGATGCCATGATGTGGAAACTTGTTGGCTTCAAATACTTTTGATTTATCTTCAAACTATGATCATGAT CTTTG
	nt 3233 - nt 3657 of X53176.1
Notes:	The Itga4 (integrin subunit a4) probe used in this study by Bajanca et al., 2004 [PMID:15366018] is described as a gift from JT Yang. Editors note: This probe is described in further detail by Pinco, Liu & Yang, 2002 [PMID:12221126] , as corresponding "to a 425bp HincII(3281)/EcoRI(3706) 3' fragment of pZ5.6 (Neuhaus et al., 1991 [PMID:1840602] )." Neuhaus submitted the mouse integrin alpha-4 mRNA sequence X53176.1 to Genbank to accompany this publication and digestion of this sequence with HincII/EcoRI identifies a fragment of the same size within the 3' UTR as described by Pinco et al.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:	4% paraformaldehyde
Staining procedure:	alkaline phosphatase + BMpurple

General Information

Authors:	Bajanca F; Luz M; Duxson MJ; Thorsteinsdottir S, 2004 [PMID:15366018] , Indexed and Spatially Mapped by EMAGE
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1002/dvdy.20136] [ PMID:15366018] Bajanca F, Luz M, Duxson MJ, Thorsteinsdottir S 2004 Integrins in the mouse myotome: developmental changes and differences between the epaxial and hypaxial lineage. Dev Dyn (231):402-15
	[ doi:10.1091/mbc.02-05-0086] [ PMID:12221126] Pinco KA, He W, Yang JT 2002 alpha4beta1 integrin regulates lamellipodia protrusion via a focal complex/focal adhesion-independent mechanism. Mol Biol Cell (13):3203-17
	[ doi:10.1083/jcb.115.4.1149] [ PMID:1840602] Neuhaus H, Hu MC, Hemler ME, Takada Y, Holzmann B, Weissman IL 1991 Cloning and expression of cDNAs for the alpha subunit of the murine lymphocyte-Peyer's patch adhesion molecule. J Cell Biol (115):1149-58
Links:	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source