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EMAGE:4425

Tubb5 tubulin, beta 5 class I ( MGI:107812)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4425
Fig7A (#9 - Tubb5). Tateossian H; Powles N; Dickinson R; Ficker M; Maconochie M, "Determination of downstream targets of FGF signalling using gene trap and cDNA subtractive approaches." Exp Cell Res 2004 Jan 1;292(1):101-14. [PMID:14720510]

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4425Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4425_wholemount_strong_3D_1.wlz
4425_wholemount_moderate_3D_1.wlz
4425_wholemount_weak_3D_1.wlz
4425_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4425_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
central nervous system
detected detected
limb
detected detected
regionalExpression was detected in the distal part of the forelimb.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3757738
Entity Detected:Tubb5, tubulin, beta 5 class I ( MGI:107812)
Notes:The Tubb5 probe (#9) used in this study by Tateossian et al., 2004 [PMID:14720510] is of unreported sequence, yet the authors state that it belongs to UniGene cluster Mm.1703, and X04663 is an identifier sequence for the gene (see Table 2 therein). Editors note: Mm.1703 has since been retired and replaced by Mm.472691 (Tubulin, beta 5).
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:(C57BL/6 x CBA)F1
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Tateossian H; Powles N; Dickinson R; Ficker M; Maconochie M, 2004 [PMID:14720510] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1016/j.yexcr.2003.08.008] [ PMID:14720510] Tateossian H, Powles N, Dickinson R, Ficker M, Maconochie M 2004 Determination of downstream targets of FGF signalling using gene trap and cDNA subtractive approaches. Exp Cell Res (292):101-14
Links:MGI:3757838 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE