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EMAGE:4427

Abcc4 ATP-binding cassette, sub-family C (CFTR/MRP), member 4 ( MGI:2443111)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4427
Supplementary Fig4K. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.ydbio.2006.05.002] Dev Biol 297: 141-157, Jukkola T; Lahti L; Naserke T; Wurst W; Partanen J, FGF regulated gene-expression and neuronal differentiation in the developing midbrain-hindbrain region. Copyright 2007

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: white arrowhead = midbrain-hindbrain boundary.
Expression Pattern Description
Spatial Annotation:
EMAGE:4427Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4427_wholemount_strong_3D_1.wlz
4427_wholemount_moderate_3D_1.wlz
4427_wholemount_weak_3D_1.wlz
4427_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4427_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
midbrain
detected detected
regionalexpression restricted to a region near the midbrain-hindbrain boundary.
eye
weak weak
regionalWeak expression was detected in the eye primordium.
rhombomere 01
detected detected
regionalexpression restricted to a region near the midbrain-hindbrain boundary, and in the most anterior R1.
olfactory pit
weak weak
Weak expression was detected in the olfatory bulb.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Abcc4 probeA
Entity Detected:Abcc4, ATP-binding cassette, sub-family C (CFTR/MRP), member 4 ( MGI:2443111)
Sequence:sense strand is shown

>Abcc4 probeA
GGGGATGGAAACTGTCATTTATTTCAGTTGACTTTGGTTTGTCCTGTGATGGTGTGAAAAATAGAAATGA
AATAGGAACATACTTGTATGACCGCTTTTAATCTCTAAGGACCAAATAAACATAAACATATATACAGTAA
GACATTTCTAAATATTCAAATAGATCTTAATATTGTCAGCATGGCATCTCACTCTCTTGAAATATCAACT
GCGACTACCAATAAAGGACGAATCTACATCATTTACTGGCCTAAACATAATTACATTCATTACAGACTTC
TGAATAC
Notes:The Abcc4 (Mrp4) probe used in this study by Jukkola et al., 2006 [PMID:16782087] was synthesized from the insert of IMAGE:1153158.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Jukkola T; Lahti L; Naserke T; Wurst W; Partanen J, 2006 [PMID:16782087] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1016/j.ydbio.2006.05.002] [ PMID:16782087] Jukkola T, Lahti L, Naserke T, Wurst W, Partanen J 2006 FGF regulated gene-expression and neuronal differentiation in the developing midbrain-hindbrain region. Dev Biol (297):141-57
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE