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EMAGE:4434

Fgf18 fibroblast growth factor 18 ( MGI:1277980)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4434
Fig1B wt Fgf18. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.ydbio.2006.05.002] Dev Biol 297: 141-157, Jukkola T; Lahti L; Naserke T; Wurst W; Partanen J, FGF regulated gene-expression and neuronal differentiation in the developing midbrain-hindbrain region. Copyright 2007

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: black arrowhead indicates midbrain-hindbrain boundary.
Expression Pattern Description
Spatial Annotation:
EMAGE:4434Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4434_wholemount_strong_3D_1.wlz
4434_wholemount_moderate_3D_1.wlz
4434_wholemount_possible_3D_1.wlz
4434_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4434_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
midbrain
detected detected
regionalExpression was detected in the midbrain-R1 region.
rhombomere 01
detected detected
Expression was detected in the midbrain-R1 region.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Fgf18 probeA
Entity Detected:Fgf18, fibroblast growth factor 18 ( MGI:1277980)
Sequence:sense strand is shown

>Fgf18 probeA
AATTCTACCTGTGTATGAACCGAAAAGGCAAGCTCGTGGGGAAGCCTGATGGTACTAGCAAGGAGTGCGT
GTTCATTGAGAAGGTTCTGGAAAACAACTACACGGCCCTGATGTCTGCCAAGTACTCTGGTTGGTATGTG
GGCTTCACCAAGAAGGGGCGGCCTCGCAAGGGTCCCAAGACCCGCGAGAACCAGCAAGATGTACACTTCA
TGAAGCGTTACCCCAAGGGACAGGCCGAGCTGCAGAAGCCCTTCAAATACACCACAGTCACCAAGCGATC
CCGGCGGATCCGCCCCACTCACCCCGGCTAGGTCCGGCCACACTCACCCCCCCAGAGAACTACATCAGAG
GAATATTTTTACATGAAAAATAAGGAAGAATCTCTATTTTTGTACATTGTGTTTAAAAGAAGACAAAAAC
TGAACCTAAAGTCTTGGGAGGAGGGGCGATAGGATTCCACTGTTGACCTGAACCCCATGACAAAGGACTC
ACACAAGGGGACCGCTGTCAACCCACAGGTGCTTGCCTCTCTCTAGGAGGTGACAATTCAAAACTCATCC
CCAGAGGAGGACTTGAACGAGGAAACTGCGAGAAACCAAAGTCCTTTCCCCCCAAAGGTTCTGAAAGCAA
ACAAAACAAAAACAAA
nt 449 - nt 1119 of NM_008005.1
Notes:The Fgf18 probe used in this study by Jukkola et al., 2006 [PMID:16782087] is indicated as that previously described by Xu et al., 2000 [PMID:10751172] i.e.: "The template for the Fgf18 probe, consisting 310 bp of 3' coding sequence and 360 bp of 3'UTR, was subcloned from a cDNA isolated from a mouse E14.5 embryonic cDNA library (Novagen, Cat. No. 69636-1)."
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Jukkola T; Lahti L; Naserke T; Wurst W; Partanen J, 2006 [PMID:16782087] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1016/j.ydbio.2006.05.002] [ PMID:16782087] Jukkola T, Lahti L, Naserke T, Wurst W, Partanen J 2006 FGF regulated gene-expression and neuronal differentiation in the developing midbrain-hindbrain region. Dev Biol (297):141-57
 [ PMID:10751172] Xu J, Liu Z, Ornitz DM 2000 Temporal and spatial gradients of Fgf8 and Fgf17 regulate proliferation and differentiation of midline cerebellar structures. Development (127):1833-43
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE