EMAGE mouse gene expression spatial database

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EMAGE:4475

Unc5b unc-5 homolog B (C. elegans) ( MGI:894703)
TS19 (11.5 dpc)
in situ hybridisation

Data Images


Figure 2B. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(02)00248-4] Mech Dev 118:191-7, Engelkamp D, Cloning of three mouse Unc5 genes and their expression patterns at mid-gestation. Copyright 2002.

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

4475_wholemount_moderate_3D_1.wlz

4475_wholemount_weak_3D_1.wlz

4475_wholemount_possible_3D_1.wlz

4475_wholemount_notDetected_3D_1.wlz

4475_wholemount_strong_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:4475_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

eye

detected

limb

detected

utricle

detected

regional

Expression in the semicircular canal.

cardiovascular system

detected

Expression in vasculature

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	Unc5b probeA
Entity Detected:	Unc5b, unc-5 homolog B (C. elegans) ( MGI:894703)
Sequence:	sense strand is shown >Unc5b probeA GCCTCAGGGTCTACTGTCTGGAGGACACACCTGTAGCACTGAAGGAGGTCCTGGAGCTGGAGAGGACTCT GGGTGGCTACTTGGTGGAGGAGCCCAAGCCTTTGCTCTTTAAGGACAGTTACCACAACCTACGCCTCTCC CTCCATGACATCCCCCATGCCCACTGGAGGAGCAAACTACTGGCCAAGTACCAGGAGATTCCCTTCTACC ACGTCTGGAATGGCAGCCAGAGAGCCCTGCACTGCACTTTCACCCTGGAGAGGCATAGCCTGGCCTCCAC GGAGTTCACCTGTAAGGTCTGCGTGCGGCAGGTCGAAGGGGAAGGCCAGATTTTCCAGCTGCACACAACG TTGGCCGAGACGCCTGCTGGCTCCCTGGATGCTCTCTGCTCTGCCCCGGGCAATGCCATCACCACCCAGC TGGGACCCTATGCCTTCAAGATACCCCTGTCCATCCGCCAAAAGATCTGCAGCAGCCTGGACGCCCCCAA CTCCCGGGGCAACGACTGGAGGCTGTTGGCGCAGAAGCTGTCCATGGACCGGTACCTAAACTACTTCGCC ACCAAAGCTAGTCCCACAGGTGTCATCTTAGACCTCTGGGAAGCTCGGCAACAGGATGACGGGGACCTCA ACAGCCTGGCCAGTGCCTTGGAGGAGATGGGCAAGAGTGAGATGCTGGTAGCCATGGCCACAGATGGCGA TTGCTGAGTGCCTGTGACCACAGGCCTGTGGGGATCAGTAGGAGACGGTGCAAGGAGGCCTGGCAGCCTC TGCACAGGGGTGCCCAGCCTCCACCACTCCTGGCTCACAGCAGGAATGGTCCTTCAACTCCCTCCCCGCC ACAACCCTCAGACCACCACCACCAGCCTTAGAAAGTCTCTGTGCTCTACTGCCAAGAGGCCGGGATCCTC TGGCCCACTGTTTCCCCAGCTCACTCTGGGGTGGGCTGAGGCCTCTGGGACAGCTGAAAGCCAGAGGCTT TCCCCTGCGACAACACACCACCCTCAGCCCTGTGACTTTGGGGACCCACAGGTTTCAATTCTGTGTTCAC ATGGTCCTGGGCTAGGGACCGCTCTCTTATCCCGGGTCGAGTTCAGTTCAGGCAAACTGCTTTTTCCTGT CCACAAGCAGAGAGGGAAGATTAGGGGAGTGGGGGTGGGGGGTGGGGGATGAGCCTCAGAAGTCAGCGAG ACTCAGGTAGTGAGAGAGCAAAAACAGTAAGGGCAAAGAAAGACCCAGTTTCTTAGGGAACGCAAATGAT TTATTATCCAGATACTTGGATAGTTCCTTTTTAAGAAAACAAAACAAACAAAAAAAAGTAAAAAAAAAAA AAAAAAAA
	nt 2451 - nt 3788 of AJ487853.1
Notes:	The Unc5b (Unc5h2) probe used in this study by Engelkamp, 2002 [PMID:12351186] covered "bp 2451-3788 of AJ487853". The pattern was verified by using at least one other non-overlapping RNA probe. Hybridisations with sense probes gave no signal.
Chemistry:	RNA
Strand:	antisense

Specimen

Organism:	mouse
Age:	11.5 dpc
Theiler Stage:	TS19
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

General Information

Authors:	Engelkamp D, 2002 [PMID:12351186] . Indexed and spatially mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1016/S0925-4773(02)00248-4] [ PMID:12351186] Engelkamp D 2002 Cloning of three mouse Unc5 genes and their expression patterns at mid-gestation. Mech Dev (118):191-7
Links:	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source