Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:4479

Pitx3 paired-like homeodomain transcription factor 3 ( MGI:1100498)
TS19 (11.5 dpc)
in situ hybridisation

Data Images
EMAGE:4479
Fig6B. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.ydbio.2006.05.002] Dev Biol 297: 141-157, Jukkola T; Lahti L; Naserke T; Wurst W; Partanen J, FGF regulated gene-expression and neuronal differentiation in the developing midbrain-hindbrain region. Copyright 2007

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: White open arrowhead indicates the midbrain-hindbrain boundary; Black arrowhead points to a region where Pitx3 expression extends posteriorly in Fgfr1 mutant embryos. Ventral brain morphology has been visualized with a red broken line.
Expression Pattern Description
Spatial Annotation:
EMAGE:4479Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4479_wholemount_possible_3D_1.wlz
4479_wholemount_notDetected_3D_1.wlz
4479_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4479_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
midbrain
detected detected
regionalExpressed in ventral midbrain, in dopaminergic neuron progenitors.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Pitx3 probeA
Entity Detected:Pitx3, paired-like homeodomain transcription factor 3 ( MGI:1100498)
Sequence:sense strand is shown

>Pitx3 probeA
GTTCCAGCCTCTCCGGACAGGGGTCGCCTAGACTGGCTTGCCCTCGTCCCAGGGTCTGAAAGGGGTGCCA
GAGCACCCGGGAAGAGGCCGCGGGCTTCGAAGAGGGCCTTTTCCCTCGCAGCCCCCGAGCGGTGGTCTGA
CCCCTATGCGGAGACCGCGCCCCTAGGACTAAGGCCAGGAACAGGGACCAGCTCCCCCAGGGCCAATTCA
CCCTTGGCTCACCCCGCCTTCTCCAGACTCCCCCTATCCCATTTTCAAAGATCAATGAAATAAACGTGCG
CGGACTGTCAAAAAAAAAAAAAAA
Notes:The Pitx3 probe used in this study by Jukkola et al., 2006 [PMID:16782087] was synthesized from the insert of IMAGE:482871.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:11.5 dpc
Theiler Stage:TS19
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Jukkola T; Lahti L; Naserke T; Wurst W; Partanen J, 2006 [PMID:16782087] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1016/j.ydbio.2006.05.002] [ PMID:16782087] Jukkola T, Lahti L, Naserke T, Wurst W, Partanen J 2006 FGF regulated gene-expression and neuronal differentiation in the developing midbrain-hindbrain region. Dev Biol (297):141-57
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE