EMAGE mouse gene expression spatial database

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EMAGE:4480

Sfrp2 secreted frizzled-related protein 2 ( MGI:108078)
TS17 (10.5 dpc)
in situ hybridisation

Data Images


Fig2L. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.ydbio.2006.05.002] Dev Biol 297: 141-157, Jukkola T; Lahti L; Naserke T; Wurst W; Partanen J, FGF regulated gene-expression and neuronal differentiation in the developing midbrain-hindbrain region. Copyright 2007

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

Image annotations: The MHB (midbrain-hindbrain boundary) is marked with a white arrowhead; Red arrow indicates a region of gene expression that is altered between wild-type and Fgfr1 mutants; di - diencephalon.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

4480_wholemount_moderate_3D_1.wlz

4480_wholemount_weak_3D_1.wlz

4480_wholemount_notDetected_3D_1.wlz

4480_wholemount_strong_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:4480_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rhombomere 01

strong

regional

Strong Sfrp2 expression was detected in the dorsal R1.

midbrain

detected

graded

In the dorsal midbrain, the expression domain was graded diminishing towards the MHB (midbrain-hindbrain boundary).

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	Sfrp2 probeA
Entity Detected:	Sfrp2, secreted frizzled-related protein 2 ( MGI:108078)
Sequence:	sense strand is shown >Sfrp2 probeA GCGCGGGCTTGCAGTGCCTTGCCCGCGCCGACCTCCCGGCGCCCGGCTTCGCGCGTTCGCGCCGCCCGCT GTCCAGAGCCCCCACGAGCAGAGCGAGGGAGTCCCGGACGAGCTCGAGCTCCGGCCGCCTCTCGCTTCCC CCGCTCGGCTCCCTCCGCCCCCCGGGGGTCGCTAGTCCACGATGCCGCGGGGCCCTGCCTCGCTGCTGCT GCTAGTCCTCGCCTCGCACTGCTGCCTGGGCTCGGCGCGTGGGCTCTTCCTCTTCGGCCAGCCCGACTTC TCCTACAAGCGCAGCAACTGCAAGCCCATCCCCGCCAACCTGCAGCTGTGCCACGGCATCGAGTACCAGA ACATGCGGCTGCCCAACCTGCTGGGCCACGAGACCATGAAGGAGGTGCTGGAGCAGGCGGGCGCCTGGAT TCCGCTGGTCATGAAGCAGTGCCACCCGGACACCAAGAAGTTCCTGTGCTCGCTCTTCGCCCCTGTCTGT CTCGACGACCTAGATGAGACCATCCAGCCGTGTCACTCGCTCTGCGTGCAGGTGAAGGACCGCTGCGCCC CGGTCATGTCCGCCTTCGGCTTCCCCTGGCCAGACATGCTGGAGTGCGACCGTTTCCCGCAGGACAACGA CCTCTGCATCCCCCTCGCTAGTAGCGACCACCTCCTGCCGGCCACAGAGGAAGCTCCCAAGGTGTGTGAA GCCTGCAAAACCAAGAATGAGGACGACAACGACATCATGGAAACCCTTTGTAAAAATGACTTCGCACTGA AAATCAAAGTGAAGGAGATAACGTACATCAACAGAGACACCAAGATCATCCTGGAGACAAAGAGCAAGAC CATTTACAAGCTGAACGGCGTGTCCGAAAGGGACCTGAAGAAATCCGTGCTGTGGCTCAAAGACAGCCTG CAGTGCACCTGTGAGGAGATGAACGACATCAACGCTCCGTATCTGGTCATGGGACAGAAGCAGGGCGGCG AGCTGGTGATCACCTCCGTGAAACGGTGGCAGAAGGGCCAGAGAGAGTTCAAGCGCATCTCCCGCAGCAT CCGCAAGCTGCAATGCTAGTTTCCCAGTGGGGTGGCTTCTCTCCATCCAGGCCCTGAGCTCTGTAGACCA CTTCCGCTCCGCGACCTCATTTCCGGTTTCCCAAGCACAGTCCGGGAAAGCTACAGCCCCAGCTTGGAGC CGCTTGCCCTGCCTCCTGCATGTGTGTATCCCTAACATGTCCTGAGTTATAAGGCCCTAGGAGGCCTTGG AAACCCATAGCTGTTTTCACGGAAAGCGAAAAGCCCATCCAGATCTTGTACAAATATTCAAACTAATAAA ATCATGACTATTTTTATGAAGTTTTAGAACAGCTCGTTTTAAGGTTAGTTTTGAATAGCTGTAGTACTTT GACCCGAGGGGCATTTTCTCTCTTTGGTCAGTCTGTTGGCTTATACCGTGCACTTAGGTTGCCATGTCAG GCGAATTGTTTCTTTTTTTTTTTTTTTTTCCCTCTGTGGTCTAAGCTTGTGGGTCCCAGACTTAGTTGAG ATAAAGCTGGCTGTTATCTCAAAGTCTTCCTCAGTTCCAGCCTGAGAATCGGCATCTAAGTCTTCAAACA TTTCGTTGCTCGTTTTATGCCCTCATGAGCTCTGACCATTGCATGCGTTCCCATCCCAGCTACAGAACTT CAGTTTATAAGCACACAGTAACCATTCCTCATTGCATGATCCCCTCAAATAAAAAGTGAATACAGTCTAT AAATTGACGAGTATTTTAAGCTTTGTTTAAAACATCTTTTAATTCAATTTTTTAATCATTTTTTTGCAAA CTAAATCATTGTAGCTTACCTGTAATATACGTAGTAGTTGACCTGGAAAAGTTGTAAAAATATTGCTTTA ACCGACACTGTAAATATTTCAGATAAACATTATATTCTTTGTATATAAACTTTACATCTTGTTGTACCTA AAAAAAAAAAAAAA
	nt 1 - nt 1974 of BC014722.1
Notes:	The Sfrp2 probe used in this study by Jukkola et al., 2006 [PMID:16782087] was synthesized from IMAGE:4487469.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	10.5 dpc
Theiler Stage:	TS17
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

General Information

Authors:	Jukkola T; Lahti L; Naserke T; Wurst W; Partanen J, 2006 [PMID:16782087] , Indexed and Spatially Mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1016/j.ydbio.2006.05.002] [ PMID:16782087] Jukkola T, Lahti L, Naserke T, Wurst W, Partanen J 2006 FGF regulated gene-expression and neuronal differentiation in the developing midbrain-hindbrain region. Dev Biol (297):141-57
Links:	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source