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EMAGE:4482

Spry2 sprouty homolog 2 (Drosophila) ( MGI:1345138)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4482
Fig1C-Spry2(wt). Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.ydbio.2006.05.002] Dev Biol 297: 141-157, Jukkola T; Lahti L; Naserke T; Wurst W; Partanen J, FGF regulated gene-expression and neuronal differentiation in the developing midbrain-hindbrain region. Copyright 2007

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: The MHB is marked with arrowhead.
Expression Pattern Description
Spatial Annotation:
EMAGE:4482Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4482_wholemount_moderate_3D_1.wlz
4482_wholemount_weak_3D_1.wlz
4482_wholemount_notDetected_3D_1.wlz
4482_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4482_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
midbrain
detected detected
regionalExpression was detected both dorsally and ventrally in the midbrain-R1 region.
rhombomere 01
detected detected
regionalExpression was detected both dorsally and ventrally in the midbrain-R1 region.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Spry2 cloneA
Entity Detected:Spry2, sprouty homolog 2 (Drosophila) ( MGI:1345138)
Sequence:sense strand is shown

>Spry2 cloneA
ATTTTAACTGATACGCTTGCTACAATTCAGCTAATGGAACCCGAAGAAGAACAACAGAAGTTGGAGACCC
ACATTGCATAAGCTAAAGCAGCCAACACTCCTAGGCGAAGAAGTTGTTCTTGTTTGTGAATAGCCCATGC
AAAATGTGTAAATTAGCAAATGGCTCCCGGACCCCTCCTTGTTTTCTCCAGAGATAATGTGCTATATTTT
TGTATATACGATAATATTTGCAACTGTGAAAAAAAAACAAGTTGTGCTATGCTTCATGGCACAGTCACAA
AATATTATACTAATATGTTGTACATTCGGAAGAATGTGAATCAATCAGTATGTTTTTAGATTGTATTTTG
CCTTACAGGAAACCTTTATTGTAAGTCTCTGATTTCCCTTTGGACTTGATGTATATTGTACAGTTACAGT
AAAATTCA
Notes:The Spry2 probe used in this study by Jukkola et al., 2006 [PMID:16782087] is indicated as that previously described by Zhang et al., 2001 [PMID:11731251] i.e.: "The following expressed sequence tag (dbEST) was used for the in situ hybridization analysis after confirming its identity by sequencing: AA222134 for Sprouty2. Anti-sense riboprobe was generated by digesting plasmid with EcoRI. Anti-sense digoxigenin-labelled probe was synthesized from linearized plasmid using T3 RNA polymerase." Editors note: the clone used as template is IMAGE:670180.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Jukkola T; Lahti L; Naserke T; Wurst W; Partanen J, 2006 [PMID:16782087] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1016/j.ydbio.2006.05.002] [ PMID:16782087] Jukkola T, Lahti L, Naserke T, Wurst W, Partanen J 2006 FGF regulated gene-expression and neuronal differentiation in the developing midbrain-hindbrain region. Dev Biol (297):141-57
 [ doi:10.1016/S0925-4773(01)00526-3] [ PMID:11731251] Zhang S, Lin Y, Itaranta P, Yagi A, Vainio S 2001 Expression of Sprouty genes 1, 2 and 4 during mouse organogenesis. Mech Dev (109):367-70
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE