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EMAGE:450

Nkx2-1 NK2 homeobox 1 ( MGI:108067)
TS12 (5 Somite no.)
in situ hybridisation

Data Images
EMAGE:450
Figure 2C of Shimamura and Rubenstein, 1997 [PMID:9226442] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
This is a doiuble stained embryo: orange staining - Shh expression; purple staining - Titf1 (Nkx2.1) expression. Only the Titf1 signal has been spatially mapped in this EMAGE entry. Image annotations: ne - neuroectoderm; pr - prosencephalon; ec - ectoderm; fg - foregut endoderm; nc - notochordal plate; hm - head mesenchyme.
Expression Pattern Description
Spatial Annotation:
EMAGE:450EMAGE:450Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context movie

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
450_voxel_strong_3D_1.wlz
450_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:450_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
future prosencephalon
detected detected
regionalNkx2.1 (Titf1) is expressed medially.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1335231
Entity Detected:Nkx2-1, NK2 homeobox 1 ( MGI:108067)
Notes:The Titf1 (Nkx2.1) probe used in this study by Shimamura & Rubenstein, 1997 [PMID:9226442] is stated as being described in Shimamura et al., 1995 [PMID:8575293] , however there is no description of the probe in the latter.
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Strain:ICR
Age:5 Somite no.
Theiler Stage:TS12
Mutations:none (wild-type)
Preparation:section
Procedures
Embedding:cryosection
Staining procedure:alkaline phosphatase
General Information
Authors:Shimamura & Rubenstein, 1997 [PMID:9226442] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:9226442] Shimamura K, Rubenstein JL 1997 Inductive interactions direct early regionalization of the mouse forebrain. Development (124):2709-18
 [ PMID:8575293] Shimamura K, Hartigan DJ, Martinez S, Puelles L, Rubenstein JL 1995 Longitudinal organization of the anterior neural plate and neural tube. Development (121):3923-33
Links:MGI:1335295 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI