EMAGE mouse gene expression spatial database

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EMAGE:4504

Klf4 Kruppel-like factor 4 (gut) ( MGI:1342287)
TS18 (11.5 dpc)
in situ hybridisation

Data Images


Fig2d. Copyright: This image is from [doi:10.1002/ar.a.10089] Ehlermann J; Pfisterer P; Schorle H, "Dynamic expression of Kruppel-like factor 4 (Klf4), a target of transcription factor AP-2alpha during murine mid-embryogenesis." Anat Rec A Discov Mol Cell Evol Biol 2003 273(2):677-80. Reprinted with permission of Wiley-Liss Inc. [PMID:12845703]

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

4504_wholemount_moderate.wlz

4504_wholemount_weak.wlz

4504_wholemount_possible.wlz

4504_wholemount_notDetected.wlz

4504_wholemount_strong.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:4504_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

nose

detected

regional

Expressed in mesenchyme of the nasal pits.

axial skeleton

detected

Expressed in mesenchyme of the developing vertebrae

4th branchial arch mesenchyme

detected

regional

Expressed in mesenchyme surrounding the fourth arch artery

1st branchial arch

detected

regional

Compared to earlier stages, expression has extended rostrocaudally to the developing eye and the aboral (lateral) side of the second and third branchial arches.

2nd branchial arch

detected

regional

Compared to earlier stages, expression has extended rostrocaudally to the developing eye and the aboral (lateral) side of the second and third branchial arches.

3rd branchial arch

detected

regional

Compared to earlier stages, expression has extended rostrocaudally to the developing eye and the aboral (lateral) side of the second and third branchial arches.

forelimb bud apical ectodermal ridge

detected

hindlimb bud apical ectodermal ridge

detected

embryo

detected

regional

Expression in the abdominal region of the ventral body.

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	Klf4 probeA
Entity Detected:	Klf4, Kruppel-like factor 4 (gut) ( MGI:1342287)
Sequence:	sense strand is shown >Klf4 probeA GTGGCCGCGACAACGGTGGGGGACACTGCTGAGTCCAAGAGCGTGCAGCCTGGCCATCGGACCTACTTAT CTGCCTTGCTGATTGTCTATTTTTATAAGAGTTTACAACTTTTCTAAGAATTTTTGTATACAAAGGAACT TTTTTAAAGACATCGCCGGTTTATATTGAATCCAAAGAAGGATCTCGGGCAATCTGGGGGTTTTGGTTTG AGGTTTTGTTTCTAAAGTTTTTAATCTTCGTTGACTTTGGGGCTCGGGTACCCCTCTCTCTTCTTCGGAC TCCGGAGGACCTTCTGGGCCCCCACATTAATGAGGCAGCCACCTGGCGAGTCTGACATGGCTGTCAGCGA CGCTCTGCTCCCGTCCTTCTCCACGTTCGCGTCCGGCCCGGCGGGAAGGGAGAAGACACTGCGTCCAGCA GGTGCCCCGACTAACCGTTGGCGTGAGGAACTCTCTCACATGAAGCGACTTCCCCCACTTCCCGGCCGCC CCTACGACCTGGCGGCGACGGTGGCCACAGACCTGGAGAGTGGCGGAGCTGGTGCAGCTTGCAGCAGTAA CAACCCGGCCCTCCTAGCCCGGAGGGAGACCGAGGAGTTCAACGACCTCCTGGACCTAGACTTTATCCTT TCCAACTCGCTAACCCACCAGGAATCGGTGGCCGCCACCGTGACCACCTCGGCGTCAGCTTCATCCTCGT CTTCCCCAGCGAGCAGCGGCCCTGCCAGCGCGCCCTCCACCTGCAGCTTCAGCTATCCGATCCGGGCCGG GGGTGACCCGGGCGTGGCTGCCAGCAACACAGGTGGAGGGCTCCTCTACAGCCGAGAATCTGCGCCACCT CCCACGGCCCCCTTCAACCTGGCGGACATCAATGACGTGAGCCCCTCGGGCGGCTTCGTGGCTGAGCTCC TGCGGCCGGAGTTGGACCCAGTATACATTCCGCCACAGCAGCCTCAGCCGCCAGGTGGCGGGCTGATGGG CAAGTTTGTGCTGAAGGCGTCTCTGACCACCCCTGGCAGCGAGTACAGCAGCCCTTCGGTCATCAGTGTT AGCAAAGGAAGCCCAGACGGCAGCCACCCCGTGGTAGTGGCGCCCTACAGCGGTGGCCCGCCGCGCATGT GCCCCAAGATTAAGCAAGAGGCGGTCCCGTCCTGCACGGTCAGCCGGTCCCTAGAGGCCCATTTGAGCGC TGGACCCCAGCTCAGCAACGGCCACCGGCCCAACACACACGACTTCCCCCTGGGGCGGCAGCTCCCCACC AGGACTACCCCTACACTGAGTCCCGAGGAACTGCTGAACAGCAGGGACTGTCACCCTGGCCTGCCTCTTC CCCCAGGATTCCATCCCCATCCGGGGCCCAACTACCCTCCTTTCCTGCCAGACCAGATGCAGTCACAAGT CCCCTCTCTCCATTATCAAGAGCTCATGCCACCGGGTTCCTGCCTGCCAGAGGAGCCCAAGCCAAAGAGG GGAAGAAGGTCGTGGCCCCGGAAAAGAACAGCCACCCACACTTGTGACTATGCAGGCTGTGGCAAAACCT ATACCAAGAGTTCTCATCTCAAGGCACACCTGCGAACTCACACAGGCGAGAAACCTTACCACTGTGACTG GGACGGCTGTGGGTGGAAATTCGCCCGCTCGGATGAACTGACCAGGCACTACCGCAAACACACAGGGCAC CGGCCCTTTCAGTGCCAGAAGTGCGACAGGGCCTTTTCCAGGTCGGACCACCTTGCCTTACACATGAAGA GGCACTTTTAAATCCCACGTAGTGGATGTGACCCACACTGCCAGGAGAGAGAGTTCAGTATTTTTTTTTC TAACCTTTCACACTGTCTTCCCACGAGGGGAGGAGCCCAGCTGGCAAGCGCTACAATCATGGTCAAGTTC CCAGCAAGTCAGCTTGTGAATGGATAATCAGGAGAAAGGAAGAGTCCAAGAGACAAAACAGAAATAC
	nt 2 - nt 1958 of U20344.1
Notes:	The Klf4 probe used in this study by Ehlermann et al 2003 [PMID:12845703] was transcribed from "a 1.9 kb fragment of the murine cDNA (Genbank accession number U20344) spanning bases 2-1958 was cloned from an E10.5 cDNA library (Invitrogen, Heidelberg, Germany) and ligated in the EcoRI site of pBS II (KS) as previously described (Pfisterer et al., 2002 [PMID:11741941] ).
Chemistry:	RNA
Strand:	antisense

Specimen

Organism:	mouse
Age:	11.5 dpc
Theiler Stage:	TS18
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

General Information

Authors:	Ehlermann J; Pfisterer P; Schorle H, 2003 [PMID:12845703] . Indexed and spatially mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1002/ar.a.10089] [ PMID:12845703] Ehlermann J, Pfisterer P, Schorle H 2003 Dynamic expression of Kruppel-like factor 4 (Klf4), a target of transcription factor AP-2alpha during murine mid-embryogenesis. Anat Rec A Discov Mol Cell Evol Biol (273):677-80
	[ doi:10.1074/jbc.M108578200] [ PMID:11741941] Pfisterer P, Ehlermann J, Hegen M, Schorle H 2002 A subtractive gene expression screen suggests a role of transcription factor AP-2 alpha in control of proliferation and differentiation. J Biol Chem (277):6637-44
Links:	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source