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EMAGE:4505

Kremen2 kringle containing transmembrane protein 2 ( MGI:1920266)
TS19 (11.5 dpc)
in situ hybridisation

Data Images
EMAGE:4505
Fig 2F. Chu et al, 2004. Canonical WNT signaling promotes mammary placode development and is essential for initiation of mammary gland morphogenesis. Development 131:4819-29 [PMID:15342465] .Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
The signal appears red-brown and mammary region expression is indicated by the arrow. Inset represents a higher magnification photograph of the region indicated by the arrow.
Expression Pattern Description
Spatial Annotation:
EMAGE:4505Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4505_wholemount_moderate_3D_1.wlz
4505_wholemount_weak_3D_1.wlz
4505_wholemount_possible_3D_1.wlz
4505_wholemount_notDetected_3D_1.wlz
4505_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4505_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
surface ectoderm
detected detected
regionalShowed elevated expression in the mammary line.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Kremen2 probeA
Entity Detected:Kremen2, kringle containing transmembrane protein 2 ( MGI:1920266)
Sequence:sense strand is shown

>Kremen2 probeA
ACTGGTCGGCTCCTCAAGGAGTCATCTACTCCCCGGATTTTCCGGATGAGTATGGACCAGACCGGAACTG
CAGCTGGGTATTGGGCCAACTGGGCGCTGTGCTAGAACTCACCTTCCGCCTCTTCGAGTTGGCTGATTCT
CGAGACCGGCTGGAGCTACGCGACGTCTCGTCCGGCAACCTACTCCGTGCCTTCGACGGCGCCCATCCGC
CGCCTCCGGGACCGCTGCGCCTGCGCACTGCTGCGCTGCTGCTCACCTTCCGCAGCGACGCAAGAGGCCA
TGCTCAAGGCTTCGCGCTCACCTACCGCGGGCTGCAGGATACAGTGGAGGGCAGAGCATCTCCAGAGGAT
TCAACTGAGAGTCTCGCAGGGGACCCCGATGGGGCTAACGCGAGCTGCAGCCCCAAGCCCGGAGCTGCAC
AGGCTTCGATAGGTGCCCGAGTCTTCTCCACCGTGACGGCCTTCTCTGTGCTGCTGCTGTTGCTCCTGTC
CCTACTGCGTTTGCTGCGTCGACGGAGCTGTCTGCTGGCTCCAGGAAAAGGGTCTCTGGCCATGGGACCT
TCCCGGGGCCCCGGGAGAAGCTGGGCTGTGTGGTACCGCCGGCCCCGAGGGGTGGCCCTGCCCTGTCCCC
CAGGGGACTCTCAGGCTGAGGGTCCTGCTGCGGGCTACCGTCCCCTGAGTGCCTCCAGCCAGAGCTCCTT
GCGCTCGCTCGTCTCTGCTCTCTGACCCCAGACGTACGTGAGTCCATCACCACTTCTGCCAGCGAGATGG
ACACCCGA
nt 843 - nt 1620 of NM_028416.1
Notes:The Kremen2 (Krm2) probe template used in this study by Chu et al., 2004. [PMID:15342465] was "synthesized by PCR of E14.5 embryo cDNA with 18 bp primers designed to amplify nucleotides 843-1620 of Krm2 cDNA (Genbank accession number NM 028416). A T7 RNA polymerase-binding site was added to 3' primers to create templates for synthesis of antisense probes and to 5' primers to create sense probe templates."
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:11.5 dpc
Theiler Stage:TS19
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Chu EY; Hens J; Andl T; Kairo A; Yamaguchi TP; Brisken C; Glick A; Wysolmerski JJ; Millar SE, 2004. [PMID:15342465] . , Indexed and spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1242/dev.01347] [ PMID:15342465] Chu EY, Hens J, Andl T, Kairo A, Yamaguchi TP, Brisken C, Glick A, Wysolmerski JJ, Millar SE 2004 Canonical WNT signaling promotes mammary placode development and is essential for initiation of mammary gland morphogenesis. Development (131):4819-29
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE