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EMAGE:4512

Lbx2 ladybird homeobox homolog 2 (Drosophila) ( MGI:1342288)
TS16 (10.5 dpc)
in situ reporter

Data Images
EMAGE:4512
Fig2B. Copyright: This image is from [doi:10.1002/dvg.20302] Wei K; Chen J; Akrami K; Sekhon R; Chen F., "Generation of mice deficient for Lbx2, a gene expressed in the urogenital system, nervous system, and Pax3 dependent tissues." Genesis 2007 45(6):361-8., and is displayed with the permission of Wiley-Blackwell who owns the Copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
This specimen is a homozygous null mutant for Lbx2, however the authors state that Lbx2 deficiency did not impair mouse development, Lbx2 null mice appeared healthy and fertile and replacement of Lbx2 by the lacZ gene provides a valuable histological marker for Lbx2-expressing cells.
Expression Pattern Description
Spatial Annotation:
EMAGE:4512Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4512_wholemount_moderate_3D_1.wlz
4512_wholemount_possible_3D_1.wlz
4512_wholemount_notDetected_3D_1.wlz
4512_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4512_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
trigeminal v ganglion
detected detected
facio-acoustic vii-viii ganglion complex
detected detected
glossopharyngeal ix ganglion
detected detected
glossopharyngeal-vagus ix-x ganglion complex
detected detected
tail
detected detected
regionalExpressed ventrally, in pre-genital tubercle region
genitourinary system
detected detected
regionalExpressed in ventral tail in region to be become external genitalia
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ reporter
Entity Detected:Lbx2, ladybird homeobox homolog 2 (Drosophila) ( MGI:1342288)
Notes:Lbx2 expression in this study by Wei et al., 2007 was assayed using a targeted Lbx2 knock-out line. The targeting vector is based on the pPNT vector modified to contain loxP sites (see open triangles in Fig 1 therein) flanking the pGK neomycin resistance cassette. The 5' arm of the targeting vector consisted of 9 kb of 5' Lbx2 flanking sequence fused to a lacZ reporter gene. The 3' arm consisted of 2 kb of 3' Lbx2 flanking sequence beginning at the 30 untranslated region of the Lbx2 gene.
Labelled with:LacZ
Specimen
Organism:mouse
Strain:either: B6.129-Lbx2tm1Fchn or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6)
Age:10.5 dpc
Theiler Stage:TS16
Mutations:Lbx2tm1Fchn/Lbx2tm1Fchn
Preparation:wholemount
Procedures
Fixation:1% formaldehyde, 0.2% glutaraldehyde, 0.02% NP-40 in PBS
General Information
Authors:Wei K; Chen J; Akrami K; Sekhon R; Chen F , 2007 [PMID:17492753] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1002/dvg.20302] [ PMID:17492753] Wei K, Chen J, Akrami K, Sekhon R, Chen F 2007 Generation of mice deficient for Lbx2, a gene expressed in the urogenital system, nervous system, and Pax3 dependent tissues. Genesis (45):361-8
Links:MGI:3712795 allele description
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE