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EMAGE:4513

Lgals3 lectin, galactose binding, soluble 3 ( MGI:96778)
TS15 (20-25 Somite no.)
in situ hybridisation

Data Images
EMAGE:4513
Fig2E. Copyright: This image is from [doi:10.1002/aja.1002030211] Fowlis D; Colnot C; Ripoche MA; Poirier F. "Galectin-3 is expressed in the notochord, developing bones, and skin of the postimplantation mouse embryo." Dev Dyn 1995 203(2):241-251, and is displayed with the permission of Wiley-Liss, Inc. who owns the Copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Scale bar = 300 micrometres.
Expression Pattern Description
Spatial Annotation:
EMAGE:4513Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4513_wholemount_moderate_3D_1.wlz
4513_wholemount_possible_3D_1.wlz
4513_wholemount_notDetected_3D_1.wlz
4513_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4513_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
notochord
detected detected
gradedThe strongest signal was around the 12th pair of somites. Expression at this stage is exclusively found in the notochord.
branchial arch
not detected not detected
homogeneousExpression at this stage is exclusively found in the notochord.
body cavity or lining
not detected not detected
homogeneousExpression at this stage is exclusively found in the notochord.
embryo ectoderm
not detected not detected
homogeneousExpression at this stage is exclusively found in the notochord.
limb
not detected not detected
homogeneousExpression at this stage is exclusively found in the notochord.
mesenchyme
not detected not detected
homogeneousExpression at this stage is exclusively found in the notochord.
organ system
not detected not detected
homogeneousExpression at this stage is exclusively found in the notochord.
skeleton
not detected not detected
homogeneousExpression at this stage is exclusively found in the notochord.
tail
not detected not detected
homogeneousExpression at this stage is exclusively found in the notochord.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:19167
Entity Detected:Lgals3, lectin, galactose binding, soluble 3 ( MGI:96778)
Sequence:sense strand is shown

>MGI:19167
CGAGACAGCTTTTCGCTTAACGATGCCTTAGCTGGCTCTGGAAACCCAAACCCTCAAGGATATCCGGGTG
CATGGGGGAACCAGCCTGGGGCAGGGGGCTACCCAGGGGCTGCCTATCCTGGGGCCTATCCAGGACAGGC
TCCTCCAGGGGCCTACCCAGGACAGGCTCCTCCAGGGGCCTATCCAGGACAGGCTCCTCCTAGTGCCTAC
CCCGGCCCAACTGCCCCTGGAGCTTATCCTGGCCCAACTGCCCCTGGAGCTTATCCTGGTCAACCTGCCC
CTGGAGCCTTCCCAGGGCAACCTGGGGCACCTGGGGCCTACCCCCAGTGCTCTGGAGGCTATCCTGCTGC
TGGCCCTTATGGTGTCCCCGCTGGACCACTGACGGTGCCCTATGACCTGCCCTTGCCTGGAGGAGTCATG
CCCCGCATGCTGATCACAATCATGGGCACAGTGAAACCCAACGCAAACAGGATTGTTCTAGATTTCAGGA
GAGGGAATGATGTTGCCTTCCACTTTAACCCCCGCTTCAATGAGAACAACAGAAGAGTCATTGTGTGTAA
CACGAAGCAGGACAATAACTGGGGAAAGGAAGAAAGACAGTCAGCCTTCCCCTTTGAGAGTGGCAAACCA
TTCAAAATACAAGTCCTGGTTGAAGCTGACCACTTCAAGGTTGCGGTCAACGATGCTCACCTACTGCAGT
ACAACCATCGGATGAAGAACCTCCGGGAAATCAGCCAACTGGGGATCAGTCGTGACATAACCCTCACCAG
CGCTAACCACGCCATGATCTAAGCCAGAAGGGGCGGCACCGAAACGCCCTGTGTGCCTTAGGAGTGGGAA
ACTTTGCATTTCTCTCTCCTTATCCTTCTTGTAAGACATCCCG
nt 1 - nt 883 of J03723.1
Notes:The Lgals3 (galectin-3) probe used in this study by Fowlis, 1995 [PMID:7655085] was transcribed from "an 883 base pair galectin 3 cDNA insert (gift of J.L. Wang, Michigan State University) (Jia and Wang, 1988 [PMID:3360772] ) [was] inserted into Bluescript KS vector". Editors note: See Fig1 of Jia and Wang for the 883nt sequence (which was submitted to GenBank as J03723.1).
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:20-25 Somite no.
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Fowlis D; Colnot C; Ripoche MA; Poirier F., 1995 [PMID:7655085] . Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1002/aja.1002030211] [ PMID:7655085] Fowlis D, Colnot C, Ripoche MA, Poirier F 1995 Galectin-3 is expressed in the notochord, developing bones, and skin of the postimplantation mouse embryo. Dev Dyn (203):241-51
 [ PMID:3360772] Jia S, Wang JL 1988 Carbohydrate binding protein 35. Complementary DNA sequence reveals homology with proteins of the heterogeneous nuclear RNP. J Biol Chem (263):6009-11
Links:MGI:3575722 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE