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EMAGE:4514

Lipg lipase, endothelial ( MGI:1341803)
TS16 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4514
Fig2A. Lindegaard ML; Nielsen JE; Hannibal J; Nielsen LB, 2005. Expression of the endothelial lipase gene in murine embryos and reproductive organs. J Lipid Res 46:439-44. [doi:10.1194/jlr.M400417-JLR200]

Expression pattern clarity: one star
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4514Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4514_wholemount_moderate_3D_1.wlz
4514_wholemount_possible_3D_1.wlz
4514_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4514_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
widespread staining is seen
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Lipg probeA
Entity Detected:Lipg, lipase, endothelial ( MGI:1341803)
Notes:The Lipg (EL) probe template used in this study by Lindegaard et al., 2005 [PMID:15576837] is described as follows: "hEL-51 and hEL-31 primers were extended with sequences for T7 and T3, respectively (5'-AATTGTAATACGACTCACTATAGGGTGCAGGCAACTTCGTGAAAG-3' and 5'-GCGCGCAATTAACCCTCACTAAAGGGTGATCCCAAGACATCGTTGAGTCCA-3') and used to amplify EL cDNA with flanking T7 and T3 sequences". Editors Note: It is unclear from the text whether mouse or human template was used in this PCR amplification (the primers were designed against human sequence).
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:10.5 dpc
Theiler Stage:TS16
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Lindegaard ML; Nielsen JE; Hannibal J; Nielsen LB, 2005 [PMID:15576837] . Indexed by and spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1194/jlr.M400417-JLR200] [ PMID:15576837] Lindegaard ML, Nielsen JE, Hannibal J, Nielsen LB 2005 Expression of the endothelial lipase gene in murine embryos and reproductive organs. J Lipid Res (46):439-44
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE