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EMAGE:4519

Lrrn1 leucine rich repeat protein 1, neuronal ( MGI:106038)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:4519 EMAGE:4519 EMAGE:4519 EMAGE:4519 EMAGE:4519
Fig3A. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.ydbio.2005.01.030] Dev Biol 281(2): 145-159, Haines BP; Gupta R; Michael Jones C; Summerbell D; Rigby PW, The NLRR gene family and mouse development: Modified differential display PCR identifies NLRR-1 as a gene expressed in early somitic myoblasts. Copyright 2005 Fig3B. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.ydbio.2005.01.030] Dev Biol 281(2): 145-159, Haines BP; Gupta R; Michael Jones C; Summerbell D; Rigby PW, The NLRR gene family and mouse development: Modified differential display PCR identifies NLRR-1 as a gene expressed in early somitic myoblasts. Copyright 2005 Fig3C. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.ydbio.2005.01.030] Dev Biol 281(2): 145-159, Haines BP; Gupta R; Michael Jones C; Summerbell D; Rigby PW, The NLRR gene family and mouse development: Modified differential display PCR identifies NLRR-1 as a gene expressed in early somitic myoblasts. Copyright 2005 Fig3i. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.ydbio.2005.01.030] Dev Biol 281(2): 145-159, Haines BP; Gupta R; Michael Jones C; Summerbell D; Rigby PW, The NLRR gene family and mouse development: Modified differential display PCR identifies NLRR-1 as a gene expressed in early somitic myoblasts. Copyright 2005 Fig3ii. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.ydbio.2005.01.030] Dev Biol 281(2): 145-159, Haines BP; Gupta R; Michael Jones C; Summerbell D; Rigby PW, The NLRR gene family and mouse development: Modified differential display PCR identifies NLRR-1 as a gene expressed in early somitic myoblasts. Copyright 2005
EMAGE:4519 EMAGE:4519 EMAGE:4519
Fig3iii. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.ydbio.2005.01.030] Dev Biol 281(2): 145-159, Haines BP; Gupta R; Michael Jones C; Summerbell D; Rigby PW, The NLRR gene family and mouse development: Modified differential display PCR identifies NLRR-1 as a gene expressed in early somitic myoblasts. Copyright 2005 Fig3iv. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.ydbio.2005.01.030] Dev Biol 281(2): 145-159, Haines BP; Gupta R; Michael Jones C; Summerbell D; Rigby PW, The NLRR gene family and mouse development: Modified differential display PCR identifies NLRR-1 as a gene expressed in early somitic myoblasts. Copyright 2005 Fig3v. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.ydbio.2005.01.030] Dev Biol 281(2): 145-159, Haines BP; Gupta R; Michael Jones C; Summerbell D; Rigby PW, The NLRR gene family and mouse development: Modified differential display PCR identifies NLRR-1 as a gene expressed in early somitic myoblasts. Copyright 2005

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: (A) planes of section i-v are shown. (B) A longitudinal bisection of the head. The arrowhead indicates the sharp boundary of ventral NLRR-1 expression. r - rhombomeres. (C) Bright field image of a higher magnification of expression in the branchial arches and hypoglossal chord. Expression in the muscle precursors is indicated by the arrowheads.
Expression Pattern Description
Spatial Annotation:
EMAGE:4519Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4519_wholemount_moderate_3D_1.wlz
4519_wholemount_weak_3D_1.wlz
4519_wholemount_notDetected_3D_1.wlz
4519_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4519_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
trunk somite
detected detected
regionalSomitic expression was first seen in the dorsal lip of the myotome where myoblasts initially arise (Fig. 3Ai). As the myotome developed NLRR-1 expression extended ventrally, at first along the anterior side and eventually along the entire width of the somite (Fig. 3Ai-iv). This pattern of expression coincides with the ventral migration of the earliest myoblasts from the dorsal lip of the somite into the myotome.
branchial arch
detected detected
regionalExpression was seen in the migrating myoblasts in the branchial arches and hypoglossal chord
future hindbrain
detected detected
regionalA longitudinal dissection through the head showed expression was restricted to a strong ventral domain in the basal part of the hindbrain that had a sharp posterior boundary (Fig. 3B, arrowhead). Further stripes of expression extended dorsally from this region along the rhombomere boundaries (Fig. 3B, brackets)
neural tube
detected detected
regionalSections through the anterior neural tube revealed strong expression restricted to a ventral domain close to the floor plate, consistent with expression in motor neurons, with weaker expression spreading dorsally (Figs. 3Aiii-v). Expression was relatively low in the posterior spinal cord (Figs. 3Ai-ii).
Annotation Validation: Submitter + EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Lrrn1 probeA
Entity Detected:Lrrn1, leucine rich repeat protein 1, neuronal ( MGI:106038)
Notes:The Lrrn1 (NLRR-1) probe used in this study by Haines et al., 2005 [PMID:15893969] "was transcribed with T7 RNA polymerase from the NLRR-1 differential display clone linearised with HindIII". Editors Note: the specific sequence of the insert of the differential display clone for Lrrn1/NLRR-1 is not described by the authors.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6 x CBA
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Haines BP; Gupta R; Michael Jones C; Summerbell D; Rigby PW, 2005 [PMID:15893969] . Indexed and spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/j.ydbio.2005.01.030] [ PMID:15893969] Haines BP, Gupta R, Jones CM, Summerbell D, Rigby PW 2005 The NLRR gene family and mouse development: Modified differential display PCR identifies NLRR-1 as a gene expressed in early somitic myoblasts. Dev Biol (281):145-59
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE