EMAGE mouse gene expression spatial database

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EMAGE:4520

Lrrn3 leucine rich repeat protein 3, neuronal ( MGI:106036)
TS17 (10.5 dpc)
in situ hybridisation

Data Images


Fig7A. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.ydbio.2005.01.030] Dev Biol 281(2): 145-159, Haines BP; Gupta R; Michael Jones C; Summerbell D; Rigby PW, The NLRR gene family and mouse development: Modified differential display PCR identifies NLRR-1 as a gene expressed in early somitic myoblasts. Copyright 2005	Fig7Ai. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.ydbio.2005.01.030] Dev Biol 281(2): 145-159, Haines BP; Gupta R; Michael Jones C; Summerbell D; Rigby PW, The NLRR gene family and mouse development: Modified differential display PCR identifies NLRR-1 as a gene expressed in early somitic myoblasts. Copyright 2005	Fig7Aii. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.ydbio.2005.01.030] Dev Biol 281(2): 145-159, Haines BP; Gupta R; Michael Jones C; Summerbell D; Rigby PW, The NLRR gene family and mouse development: Modified differential display PCR identifies NLRR-1 as a gene expressed in early somitic myoblasts. Copyright 2005	Fig7Aiii. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.ydbio.2005.01.030] Dev Biol 281(2): 145-159, Haines BP; Gupta R; Michael Jones C; Summerbell D; Rigby PW, The NLRR gene family and mouse development: Modified differential display PCR identifies NLRR-1 as a gene expressed in early somitic myoblasts. Copyright 2005	Fig7Aiv. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.ydbio.2005.01.030] Dev Biol 281(2): 145-159, Haines BP; Gupta R; Michael Jones C; Summerbell D; Rigby PW, The NLRR gene family and mouse development: Modified differential display PCR identifies NLRR-1 as a gene expressed in early somitic myoblasts. Copyright 2005

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

Image annotations: approximate section planes of Ai-Ai are shown in A. drg-dorsal root ganglia; tg-trigeminal ganglia; fa-facioacoustic ganglia; gc-germ cells; ob-olfactory bulb; oc-optic cup; hb-hindbrain.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

4520_wholemount_moderate_3D_1.wlz

4520_wholemount_notDetected_3D_1.wlz

4520_wholemount_strong_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:4520_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

trigeminal v ganglion

strong

facial vii ganglion

strong

acoustic viii ganglion

strong

dorsal root ganglion

detected

neural tube

detected

hindbrain

detected

Expression in the hindbrain was restricted to a subregion in the outer edge of the neural tissue (Fig. 7Aii)

eye

detected

nose

detected

Small domains of expression are found in the head associated with the olfactory system.

primordial germ cell

strong

strong expression in the migrating germ cells in the tail

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	Lrrn3 probeA
Entity Detected:	Lrrn3, leucine rich repeat protein 3, neuronal ( MGI:106036)
Sequence:	sense strand is shown >Lrrn3 probeA GCTGTGACTGGAATCCTTAAGGGCCTGTTAACTTTCTGAAGACAAAAGCTAAGATGAAGGACACGCCACT CCAAGTCCATGTGCTACTTGGCCTAGCTATCACTACACTAGTACAAGCTATAGATAAAAAAGTGGATTGC CCCCAATTATGTACCTGTGAGATCAGGCCTTGGTTTACCCCGAGATCCATCTATATGGAAGCATCGACAG TGGACTGTAATGATTTAGGGCTTTTAAACTTCCCAGCCAGATTGCCTGCCGACACACAGATTCTGCTCCT ACAGACTAACAATATTGCAAGAATTGAACATTCCACAGACTTCCCAGTGAACCTGACTGGCCTGGACTTA TCTCAAAACAATTTATCTTCAGTCACTAATATTAATGTACAAAAGATGTCTCAGCTTCTTTCTGTGTACC TAGAGGAAAACAAGCTAACTGAGCTGCCGGAAAAGTGTCTATATGGACTGAGCAACTTGCAGGAACTCTA CGTTAATCACAACCTGCTCTCTACCATTTCTCCCGGAGCCTTCATTGGCCTACATAATCTTCTCCGGCTT CATCTCAACTCAAACAGACTGCAGATGATCAACAGTCAATGGTTTGATGCTCTTCCCAATCTCGAGATTC TGATGCTTGGGGACAACCCCATCATCAGGATCAAGGACATGAACTTTCAGCCTCTTGTCA
	nt 484 - nt 1173 of NM_010733.2
Notes:	The Lrrn3 (NLRR-3) probe used in this study by Haines et al., 2005 [PMID:15893969] is described as follows: "An NLRR-3 probe was transcribed with T3 RNA polymerase from LR3 HIII/RI A, a plasmid constructed by digesting the NLRR-3 cDNA with EcoRI and HindIII and ligating the most 5' 689 bp into pBluescript II KS, which was linearised with EcoRI." Editors Note: restriction digestion of the Lrrn3 mouse cDNA RefSeq NM_010733.2 with EcoRI and HindII identifies only two cut sites at nt1173 (HindIII) and nt1737 (EcoRI). The "most 5' 689bp EcoRI-HindIII" fragment eluded to by the authors is presumed to extend from a linker derived EcoRI site at nt 484 to the HindIII site at nt 1173.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6 x CBA
Age:	10.5 dpc
Theiler Stage:	TS17
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

General Information

Authors:	Haines BP; Gupta R; Jones C; Summerbell D; Rigby PW, 2005 [PMID:15893969] . Indexed and spatially mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1016/j.ydbio.2005.01.030] [ PMID:15893969] Haines BP, Gupta R, Jones CM, Summerbell D, Rigby PW 2005 The NLRR gene family and mouse development: Modified differential display PCR identifies NLRR-1 as a gene expressed in early somitic myoblasts. Dev Biol (281):145-59
Links:	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source